In recent years there has been considerable interest in the synthesis and separation of enantiomers of organic compounds especially because of their importance in the biochemistry and pharmaceutical industry. High-performance liquid Chromatography is a very useful method for the direct separation of enantiomers. However, about 30−40 years ago, commercially available chiral stationary phases wer...

Two single-stranded nucleic acid-binding proteins, UP1 and UP2, that were originally reported by Herrick and Alberts (Herrick, G., and Alberts, B. (1976) J. Biol. Chem. 251,2124-2132) have been purified to apparent homogeneity from calf thymus by high performance liquid chromatography. The amino acid sequence of UP1 (Williams, K. R., Stone, K. L., LoPresti, M. B., Merrill, B. M., and Planck, S....

istry 204: 215}219. Chaplin MF and Kennedy JF (eds) (1994) Carbohydrate Analysis:A Practical Approach, 2nd edn, pp. 15, 41, 66, 152, 209. Oxford: IRL Press. Charlwood J, Birrell H, Tolson D and Camilleri P (1998) Two-dimensional chromatography in the analysis of complex glycans from transferrin. Analytical Chemistry 70: 2530}2535. Guille GR, Rudd PM, Wing DR, Prime SB and Dwek RA (1996) A rapid...

From the chemical point of view, wines are aqueous alcoholic solutions containing more than 1000 components that can be present at high concentrations (g L ), but also at trace levels (ng L ). Some of these components determine the organoleptic properties of wines, while others are signi"cant for classifying their origin and/or for checking whether some adulteration has taken place. Concentrati...

In this month’s “Column Watch,” Ron Majors discusses advances in multidimensional chromatography and comprehensive multidimensional chromatography. These techniques are well suited for the separation of complex samples. Although GC GC is fairly well developed, LC LC is in its infancy. But with fast LC columns, based on monoliths, superficially porous particles, and short sub-2m particle columns...