Dietary γ-glutamyl peptides (γ-GPs) found in dry edible beans exhibit biological activities (antioxidant, anti-inflammatory) with potential benefits against chronic metabolic disorders. γ-GPs are responsible for the desirable Kokumi flavor through allosteric activation of Calcium Sensing Receptor (CaSR) present multiple mammalian tissues. In this study, we investigated effects valine (γ-EV) dia...

The liver has the remarkable ability to respond to injury with repair and regeneration. The protozoan parasite Entamoeba histolytica is the major cause of liver abscess worldwide. We report a transcriptional analysis of the response of mouse liver to E. histolytica infection, the first study looking at acute liver infection by a non-viral pathogen. Focusing on early time points, we identified 7...

Two 70-day feeding trials were conducted to reevaluate the necessity of cholesterol supplementation in diets two important marine fish species, turbot and tiger puffer. Graded levels (0, 0.5, 1.0, 2.0, 4.0% dry matter) added into basal diet (with 30% fishmeal but no oil) make five experimental diets, which designated as control, CHO-0.5, CHO-1.0, CHO-2.0, CHO-4.0, respectively. The dietary conc...

aim : in this study, to clarify the smad4blocking impact on fibrosis process, we investigated its down-regulation by shrna on activated human lx-2 cell, in vitro.   background : liver fibrosis is a critical consequence of chronic damage to the liver that can progress toward advanced diseases, liver cirrhosis and hepatocellular carcinoma (hcc).  different smad proteins play as major mediators in...

Drug-induced liver injury (DILI) is the serious and fatal drug-associated adverse effect, but its incidence is very low and individual variation in severity is substantial. Acetaminophen (APAP)-induced liver injury accounts for >50% of reported DILI cases but little is known for the cause of individual variations in the severity. Intrinsic genetic variation is considered a key element but the i...

OBJECTIVES We aimed at identifying genes related to hereditary type 2 diabetes expressed in the liver and the adipose tissue of spontaneous diabetic gerbils using suppression subtractive hybridization (SSH) screening. METHODS Two gerbil littermates, one with high and the other with normal blood glucose level, from our previously bred spontaneous diabetic gerbil strain were used in this study....

Fetal liver intriguingly consists of hepatic parenchymal cells and hematopoietic stem/progenitor cells. Human fetal liver aged 22 wk of gestation (HFL22w) corresponds to the turning point between immigration and emigration of the hematopoietic system. To gain further molecular insight into its developmental and functional characteristics, HFL22w was studied by generating expressed sequence tags...

Abstract Hyperlipidemia is a risk factor for cardiovascular disease, and miR-21a-5p plays an important role in the occurrence progression of hyperlipidemia. Here, we aimed to investigate mechanism aerobic exercise improved hyperlipidemia through enhancing expression. In this study, high-fat/high-cholesterol diet mice received 8 weeks intervention, then collected plasma liver samples, found that...

A method for the isolation of structural genes, whose transcripts do not contain terminal poly(A) sequences, is presented. Poly(A) tails of predicted length were synthesized at the 3'-OH ends of RNA molecules employing Escherichia coli ATP : RNA adenyltransferase (EC 2.7.7.19). The gene isolation was performed in two steps: (a) enrichment of DNA fragments carrying the genes of interest, (b) sub...