R. Sch€ onherr, M. Klinge, J. M. Rudolph, K. Fita, D. Rehders, F. L€ ubber, S. Schneegans, I. V. Majoul, M. Duszenko, C. Betzel, A. Brandariz-Nu~ nez, J. Martinez-Costas, R. Duden, and L. Redecke Institute of Biology, Center for Structural and Cell Biology in Medicine, University of L€ ubeck, Ratzeburger Allee 160, 23562 L€ ubeck, Germany Institute of Biochemistry, Center for Structural and Cel...

Hammar, P. 2013. lac of Time: Transcription Factor Kinetics in Living Cells. Acta Universitatis Upsaliensis. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1046. 74 pp. Uppsala. ISBN 978-91-554-8674-7. Gene regulation mediated by transcription factors (TFs) is essential for all organisms. The functionality of TFs can largely be described by t...

To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer (FRET)-based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetyla...

Despite its great industrial importance, many aspects of free radical polymerization (FRP)1 remain poorly understood. One of the few methods that can directly probe propagating radicals, i.e., living chains which are at the heart of FRP, is electron paramagnetic resonance (EPR), a very sensitive and nondestructive technique that provides information on the local structure and dynamics and the n...

The Living Labs for Information Retrieval Evaluation (LL4IR) initiative have provided a novel framework for evaluating retrieval models that involve real users. In this position paper, we propose an extension to the LL4IR framework that enables to evaluate real-time IR.

A library of fluorescently labeled protein kinase C (PKC) peptide substrates was prepared to identify a phosphorylation-induced reporter of protein kinase activity. The lead PKC substrate displays a 2.5-fold change in fluorescence intensity upon phosphorylation. PKC activity is readily sampled in cell lysates containing the activated PKCs. Immunodepletion of conventional PKCs from the cell lysa...

Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments--including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes--that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being s...

Demonstrating hybridization between an antisense oligodeoxynucleotide and its mRNA target has proven to be extremely difficult in living cells. To address this fundamental problem in antisense research, we synthesized "molecular beacon" (MB) reporter oligodeoxynucleotides with matched fluorescent donor and acceptor chromophores on their 5' and 3' ends. In the absence of a complementary nucleic ...

BACKGROUND Long-range oscillations of the mammalian cell proliferation rate are commonly observed both in vivo and in vitro. Such complicated dynamics are generally the result of a combination of stochastic events and deterministic regulation. Assessing the role, if any, of chaotic regulation is difficult. However, unmasking chaotic dynamics is essential for analysis of cellular processes relat...

Regime shifts from one ecological state to another are often portrayed as sudden, dramatic, and difficult to reverse. Yet many regime shifts unfold slowly and imperceptibly after a tipping point has been exceeded, especially at regional and global scales. These long, smooth transitions between equilibrium states are easy to miss, ignore, or deny, confounding management and governance. However, ...