Creatine kinase (CK) is located in an isoenzyme-specific manner at subcellular sites of energy production and consumption. In muscle cells, the muscle-type CK isoform (MM-CK) specifically interacts with the sarcomeric M-line, while the highly homologous brain-type CK isoform (BB-CK) does not share this property. Sequence comparison revealed two pairs of lysine residues that are highly conserved...

Because creatine kinase (CK) appears in cardiac lymph after myocardial infarction, this study was undertaken to determine whether lymph inactivates CK in vitro and whether interruption of cardiac lymph flow influences estimation of infarct size based on plasma CK changes in conscious dogs. After the effects of incubation of canine myocardial CK in native, deproteinized, or sulfhydrylfortified l...

Creatine kinase (CK) BB, a member of the CK gene family, is a predominantly cytosolic CK isoform in the brain and plays a key role in regulation of the ATP level in neural cells. CK BB levels are reduced in brain regions affected by neurodegeneration in Alzheimer's disease (AD), Pick's disease, and Lewy body dementia, and this reduction is not a result of decreased mRNA levels. This study demon...

Creatine kinase (CK) is located in an isoenzyme-specific manner at subcellular sites of energy production and consumption. In muscle cells, the muscletype CK isoform (MM-CK) specifically interacts with the sarcomeric M-line, while the highly homologous brain-type CK isoform (BB-CK) does not share this property. Sequence comparison revealed two pairs of lysine residues that are highly conserved ...

The total energies of atoms and with atomic number Z from 1 to 92 and singly-charge cations with Z from 2 to 92 have been calculated to an accuracy of 1 μHartree within four variants of the Kohn-Sham local-density approximation. The approximations considered are: the local-density approximation, the local-spin-density approximation, the relativistic local-density approximation, and the scalar-r...

We present nearly local definitions of correlation energy density, and its potential and kinetic components, and evaluate them for several two-electron systems. This information should provide valuable guidance in constructing better correlation functionals than those in common use. In addition, we demonstrate that the quantum chemistry and the density-functional definitions of the correlation ...

Sir, In the case of American cutaneous leishmaniasis, the risk of serious morbidity from cutaneous and mucocutaneous forms requires highly effective parenteral therapy. Pentavalent antimony compounds have been the mainstay of antileish-manial therapy for half a century. However, despite high effectiveness, these drugs require long duration regimens and cause frequent and serious toxic effects. ...

Sir, Creatine Kinase (CK) E.C. 2.7.3.2. is separable into three different isoenzymes by a number of methods. Each isoenzyme is a dimer composed of two subunits (Bor M) each having a relative molecular mass of 40,000. These isoenzymes are widely distributed in tissues, CK-MM and CK-MB are found mainly in the skeletal and heart muscles and CK-BB in G.l. tract and the brain with small amounts also...

We used an enzyme-linked immunoabsorbent assay to measure creatine kinase (CK; EC 2.7.3.2) BB in the sera of 58 cancer patients. A pre-incubation step with an anti-CK-M antibody-coated bead removed M chain components, and CK-BB was quantified with use of an anti-CK-B antibody-coated tube. No cross reactivity was observed with mitochondrial CK or CK-MM; CK-MB cross reacted slightly (1.6%). Macro...

The expression of cytokeratins (CKs) 8, 18 and 19 was analyzed in male and female rat gonads from the undifferentiated stage (12.5 days of gestation) until two weeks after birth by indirect immunofluorescence, using specific monoclonal antibodies anti-CK 8 (LE41), anti-CK 19 (LP2K) and anti-CK 18 (LE65 and RGE53). In the undifferentiated blastema, the somatic cells were stained for CK 8 and CK ...