The metabolism of [(14)CH(3)]2-(2,4-dichlorophenoxy)isobutyric acid (DIB) was studied in plants and cell suspension cultures of Lycopersicon esculentum Mill. sp. ;Lukullus'. Both plants and cells in suspension culture showed a rapid uptake of DIB from nutrient media. The metabolites, isolated by extraction with methanol and separated by chromatographic methods, were identified by enzymic, chemi...

Highly purified isolates of potato spindle tuber viroid (PSTV) from Lycopersicon esculentum cv. Rutgers and citrus exocortis viroid (CEV) from Gynura aurantiaca have been compared. Two-dimensional fingerprinting analysis of PSTVand CEV-RNA labeled in vitro with lzrI has demonstrated that (i) each of these RNA species has a complexity compatible with the size estimate of 250X350 nucleotides and ...

Color change, a measure of the ripening of pericarp disks of tomato fruits (Lycopersicon esculentum Mill. cv. Moneymaker), was delayed by osmotic water uptake. An even greater delay occurred when substances from the disks were allowed to leach out or to diffuse into agar, indicating the existence of a water-soluble substance(s) necessary for the ripening process. Osmotic solutions, allowing for...

The intracellular location of ADP-glucose pyrophosphorylase (AGP) in developing pericarp of tomato (Lycopersicon esculentum Mill) has been investigated by immunolocalization. With the use of a highly specific anti-tomato fruit AGP antibody, the enzyme was localized in cytoplasm as well as plastids at both the light and electron microscope levels. The immunogold particles in plastids were locali...

The ProMEX database is one of the main collection of annotated tryptic peptides in plant proteomics. The main objective of the ProMEX database is to provide experimental MS/MS-based information for cell type-specific or sub-cellular proteomes in Arabidopsis thaliana, Medicago truncatula, Chlamydomonas reinhardtii, Lotus japonicus, Lotus corniculatus, Phaseolus vulgaris, Lycopersicon esculentum,...

The SPINDLY (SPY) gene is a crucial component of the gibberellin signal transduction pathway in Arabidopsis thaliana (L.) Heynh. In this study, the cloning of the SPINDLY-orthologous gene (LeSpy) from tomato (Lycopersicon esculentum Mill.) and its characterization are reported. SPY and LeSpy show high sequence similarity along their entire lengths, which is reflected in the conservation of exon...

Developmental stages of Meloidogyne javanica were successfully released from roots by treatment with commercially available cellulase and pectinase. The average percentage recovery of nematode developmental stages from Dolichos lablab, Elymus glaucus, and Lycopersicon esculentum were as follows: eggs = 526%, J2 = 272%, J3 = 783%, J4 = 549%, adult females = 285%, and total = 425%, expressed as p...

Mature green tomato fruit (Lycopersicon esculentum Mill. cv ;Rutgers') were infiltrated with 0.5 milliliters of sterilized, distilled water alone, or containing enough glucose or galactose to allow for a concentration of 400 micrograms per gram fresh weight after infiltration. All fruit showed a transient peak of ethylene production within 6 hours due to the slight wounding by vacuum infiltrati...

Lycopene extract from tomato is a lycopene-rich extract prepared from the ripe fruits of tomato (Lycopersicon esculentum L.). The product is manufactured by crushing tomatoes, to produce crude tomato juice that is then separated into serum and pulp. The pulp is subsequently extracted using ethyl acetate as a solvent. The final extract consists of tomato oil in which lycopene together with a num...