Viral antigen prepared by heat inactivation of vaccinia virus stimulated production of interferon in association with transformation of sensitized human lymphocytes in vitro. Involvement of a macrophage-lymphocyte interaction in production of interferon stimulated by viral antigen was found in which macrophage greatly augmented the amount of interferon produced by lymphocytes. Reimmunization wi...

BACKGROUND It hasn't been clearly understood yet whether sensitization to antibiotics, the virus itself or transient loss of drug tolerance due to the virus, is responsible for the development of maculopapular exanthems following amoxicillin intake in patients with infectious mononucleosis. We aimed to examine whether sensitization to penicillin developed among patients with skin rash following...

I would like to comment on the letter from Dessau et al.: “Lymphocyte transformation test for diagnosis of Lyme borreliosis is currently not documented to be clinically useful” [1]. I wish to thank Dessau and colleagues for their interest in our study and am very pleased that they confirm that the lymphocyte transformation test (LTT) shows a high specificity in seronegative cases [1]. Especiall...

A positive lymphocyte transformation test to beta-lactams (beta-L) was found in 12 of 29 subjects with adverse drug reaction (ADR) to beta-L, irrespective of either the type of clinical manifestation or the presence of specific serum IgE. Short-term T cell lines specific for penicillin G, amoxicillin, and ampicillin could be generated only from subjects with ADR (eight with positive and one wit...

UNLABELLED The purpose of research is to study the influence of plant mitogens and antigens of the water-salt extract from the bodies of Hirudo verbana, H. medicinalis, H. orientalis on the reaction of lymphocyte blast-transformation and the synthesis of pro-inflammatory cytokines (IL-1β, IL-8, TNF-α) in patients' cell culture supernatants before and after hirudotherapy. RESEARCH METHODS the ...

In vitro tests of lymphocyte function have been performed in 61 patients with ;classical' or ;definite' rheumatoid arthritis. In vitro lymphocyte function was assessed by lymphocyte transformation responses to phytohaemagglutinin (PHA), Pokeweed mitogen (PWM), Candida antigen, and herpes simplex type I (HSV1). Follow up data were available after 6 months of treatment in 32 of these patients. Sp...

Five mongrel dogs were sensitized to tuberculoprotein with Freund adjuvant, as determined by responses to tuberculin skin tests and by in vitro lymphocyte cultures. These animals served as blood donors for production of transfer factor. Nine mongrel dogs received single doses of transfer factor that was tuberculin specific. Successful transfer of tuberculin sensitivity as measured by lymphocyte...

OBJECTIVE The aim of the current study is to test whether immortalized B-lymphocyte cell line via Ebstein-Barr virus (EBV) transformation is feasible and can be an unlimited source of genome wide study. METHODS We obtained peripheral whole blood from 5 ovarian cancer patients and immortalized the B-cell lines using EBV transformation. The success rate was analyzed and the bio-identity of the ...

There is evidence that general anaesthesia interferes with the immune response in man (Bruce and Wingard, 1971). It may affect postoperative infections, anaphylaxis, response to organ transplantation and tumour growth. It has been speculated that there is a relationship between this immunosuppression and the development of postoperative hepatitis (Bruce and Wingard, 1971). Anaesthetists appear ...

A 15-year-old male admitted for Pott's puffy tumor developed recurrent episodes of fever, diffuse morbilliform rash, eosinophilia, and tubulointerstitial nephritis while on multiple antibiotics. Lymphocyte blast transformation (LBT), a method of detecting cellular immune response by measuring levels of interferon-γ (IFN-γ), was used to diagnose vancomycin hypersensitivity and guide antibiotic s...