نتایج جستجو برای: lysing agent
تعداد نتایج: 253877 فیلتر نتایج به سال:
Deep-water stratigraphic successions from syn- to post-rift stages are an archive of evolving physiographic configurations, and can record axial transverse sedimentary sources. The healing topography decreases the influence syn-rift structures on sedimentation patterns transport processes over time, which leads a long-term transition dominantly dispersal patterns. Halten Dønna terraces, offshor...
Inactivation mechanism of supercritical carbon dioxide (SC-CD) combination with Thymbra spicata essential oil (TEO) treatments on bacteria in tryptone soy broth (TSB) and juices was investigated. Salmonella Typhimurium significantly inactivated by 3.45 5.55 logs after SC-CD+TEO treatment TBS for 35 min at 7.6 10.13 MPa respectively. Klebsiella pneumoniae showed higher resistance to than other t...
To correlate viability with attachment capacity, Mycoplasma gallisepticum cell harvested at different growth phases and treated by various agents were tested for their capacity to attach to human erythrocytes. The results show that viability per se is not essential for M. gallisepticum attachment to erythrocytes, as cells killed by ultraviolet irradiation anmd membranes isolated by lysing M. ga...
Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects the...
Morse, M. L. (University of Colorado Medical Center, Denver) and J. W. LaBelle. Characteristics of a staphylococcal phage capable of transduction. J. Bacteriol. 83:775-780. 1962-The growth of staphylococcal phage 53, a temperate phage, has been studied. The latent periods in nutrient broth are: infected sensitive cells, 45 min; ultraviolet-induced lysogenic cells, 83 min. The burst size, as ind...
To obviate the well-documented problem of hematocrit dependency of the Yellow Springs Instruments (YSI) whole-blood lactate analyzer, we modified the dilution buffer by including a lysing reagent. This makes the results comparable with those of methods performed with deproteinized whole-blood samples. No centrifugation step is needed, thus preserving the convenience of the YSI instrument for st...
Stimulation of human vaccinia virus immune peripheral blood mononuclear cells in vitro from vaccinia virus-immune donors with live vaccinia virus-infected autologous cells generated vaccinia virus-specific cytotoxic T lymphocytes (CTL) capable of lysing vaccinia virus-infected cells. We generated vaccinia virus-specific CD8+ clones and CD4+ CTL lines by limiting dilution from two donors by usin...
Crude concentrates of epidermal chalone from pig skin in some cases have shown striking oncolytic activity in melanomata in hamsters and mice. Attempts to purify this antitumor factor by biochemical methods failed. A correlation of epidermal or melanocytic chalone activity in vitro and oncolytic activity in vivo was absent. From fluid collected from lysing tumors spore-forming bacteria could be...
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