some specific and non-specific immune variables of rainbow trout were assessed following vaccination of fish with formalin killed cells (fkc) and fkc containing extra cellular products (ecp) of s. iniae. rainbow trout weighing 80-100 g were vaccinated by intraperitoneal (i.p), dip and oral routes using fkc and fkc plus ecp with or without fround adjuvant (fa) at 16-17°c.antibody titration, lyso...

This work analyses the chitin-binding and catalytic domains of the human macrophage chitotriosidase and investigates the physiological role of this glycoside hydrolase in a complex mechanism such as the innate immune system, especially its antifungal activity. Accordingly, we first analyzed the ability of its chitin-binding domain to interact with chitin embedded in fungal cell walls using the ...

The photo-oxidation of lysozyme (hen’s egg white), sensitized by methylene blue in 84% acetic acid solution, causes the selective modification of the methionyl residues, which are converted to the sulfoxide. The photo-oxidized protein shows a drastically reduced enzymic activity. The data obtained by comparing the sensitivity to proteolytic enzymes, as. well as the accessibility of disulfide bo...

The antimicrobial peptide lysozyme is an important factor of innate immunity and exerts high potential of antibacterial activity. In the present study we evaluated the lysozyme expression in serum of multiple injured patients and subsequently analyzed their possible sources and signaling pathways. Expression of lysozyme was examined in blood samples of multiple trauma patients from the day of t...

To explore the antioxidant potential of hen egg white lysozyme N-acetylmuramide Glycan hydrolase in full fat Mozzarella cheese, an experiment was conducted where control sample brined without addition lysozyme; while four samples were along with 0.02%;w/v (T1), 0.04%;w/v (T2), 0.06%;w/v (T3) and 0.08%;w/v (T4) lysozyme. The cheese measured through several methods, including 2, 2-azino-bis (3-et...

I . Lysozyme activity was estimated in plasma and leucocytes of twelve children suffering from kwashiorkor, thirteen children with ocular signs of vitamin A deficiency and ten apparently normal children acting as controls. 2. The results showed that the activity of lysozyme in leucocytes was significantly reduced in children with kwashiorkor and in vitamin A-deficient children. Following therap...

Isolation of pathogenic or opportunistic bacteria from surrounding environment, food products, animals and humans requires establishing their potential potential. In this work, the authors have studied 109 cultures belonging to species Serratia marcescens which: 61 museum cultures, 29 isolated small ruminants environmental objects, 19 dairy products produced on private farmsteads in Chechen Rep...

T4 lysozyme (T4L) is an important model system for investigating the relationship between protein structure and function. Despite being extensively studied, a reliable, quantitative activity assay for T4L has not been developed. Here, we present an improved T4L turbidity assay as well as an affinity-based T4L expression and purification protocol. This assay is designed for 96-well format and ut...

Aminopropyl-functionalized SBA-15 mesoporous silica was used as a support to adsorb myoglobin. Then, in order to avoid the leakage of adsorbed myoglobin, lysozyme was covalently tethered to the internal and external surface of the mesoporous silica with glutaraldehyde as the coupling agent. The property of amino-functionalized mesoporous silica was characterized by N(2) adsorption-desorption an...

OBJECTIVE Estimate the contribution of monocytes/macrophages to the disease process in rheumatoid arthritis (RA), by measuring the serum levels of the leucocyte-derived granular proteins: lysozyme, myeloperoxidase (MPO), lactoferrin and human neutrophil lipocalin (HNL). METHODS Serum levels of these granular proteins were measured in patients with RA (n=23) and in healthy controls (n=27), and...