Biosynthesis of C(30) carotenoids is relatively restricted in nature but has been described in Staphylococcus and in methylotrophic bacteria. We report here identification of a novel gene (crtNb) involved in conversion of 4,4'-diapolycopene to 4,4'-diapolycopene aldehyde. An aldehyde dehydrogenase gene (ald) responsible for the subsequent oxidation of 4,4'-diapolycopene aldehyde to 4,4'-diapoly...

The bacterial inactivation kinetics of dialdehyde starch (DAS) aqueous suspension was studied by the relationship between the minimal lethal concentration (MLC) and the inactivation time at four different temperatures. The relationship between MLC and exposure time was found to follow the first-order Chick-Watson law. This first-order inactivation kinetics was modeled by pseudo-first order chem...

The important and increasing application of aluminum motivated us to determine whether and in what way does the antioxidative status of erythrocytes in aluminum acute poisoned animals change by studying the metabolism of superoxid dismutase (SOD), glutathione reductase (GR), reduced glutathione (GSH) and malonil dialdehyde (MDA). The experiment involved desert mice (gerbils) who received intrap...

Recently we described a method for the determination of alanine based upon its conversion into acetaldehyde by the action of ninhydrin (1). A method for the measurement of glycine has been devised which involves its conversion by ninhydrin to formaldehyde; the latter is then measured by its reaction with chromotropic acid. Earlier methods for the measurement of glycine are less sensitive and sp...

Deoxyadenosine (dAdo) and deoxyguanosine (dGuo) decrease methionine synthesis from homocysteine in cultured lymphoblasts; because of the possible trapping of 5-methyltetrahydrofolate this could lead to decreased purine nucleotide synthesis. Since purine deoxynucleosides could also inhibit purine synthesis de novo at an early step not involving folate metabolism, we measured in azaserine-treated...

Oxidation of 1,N6-ethenoadenosine monophosphate (epsilon AMP) with periodate cleaved the cis-diol of the ribose ring and resulted in the formation of a dialdehyde derivative (epsilon AMP-dial). At room temperature epsilon AMP-dial was unstable and underwent beta-elimination to give 4',5'-anhydro-1,N6-ethenoadenosine dialdehyde acetal (A epsilon Ado-dial). These nucleotide analogues were found t...

In addition to good mechanical properties needed for three-dimensional tissue engineering, the combination of alginate dialdehyde, gelatin and nano-scaled bioactive glass (45S5) is supposed to combine excellent cellular adhesion, proliferation and differentiation properties, good biocompatibility and predictable degradation rates. The goal of this study was to evaluate the in vitro and in vivo ...

Photosynthesis is not required for the toxicity of diphenyl ether herbicides, nor are chloroplast thylakoids the primary site of diphenyl ether herbicide activity. Isolated spinach (Spinacia oleracea L.) chloroplast fragments produced malonyl dialdehyde, indicating lipid peroxidation, when paraquat (1,1'-dimethyl-4,4'-bipyridinium ion) or diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] were ad...

Background & Aim: Osmotic stress in plants lead to increase free radicals and plants use different mechanisms to scavenge these radicals. So, researchers apply stresses especially drought stress to increase secondary metabolites such as phenolics, and antioxidants. One of the valuable medicinal plants is yarrow (Achillea tenuifolia) which is a perennial plant with different medicinal a...

It has been postulated that hepatotoxicity of carbon tetrachloride is related to its prooxidant effect on lipid peroxidation (1-8). DIANZANI and UGAZIO (8) reported that several antioxidants, i.e., propyl gallate, N, N•L-diphenyl-p-phenyl enediamine, and reduced glutathione, which are administered to rats prior to carbon tetrachloride, inhibit significantly the accumulation of hepatic triglycer...