Lithium content does not underlie the bioactive effects of LAPONITE® on human bone marrow stromal cell osteogenic activity.

High-dose chemotherapy and autologous stem cell transplantation (SCT) have become an effective care for many patients with hematological malignancies. Harvesting the stem cells is one the most important parts of SCT. The two most commonly used mobilization regimens are the use of granulocyte colony-stimulating factor (G-CSF) or G-CSF plus chemotherapy. However, about 10-30% of patients are unab...

High-dose chemotherapy and autologous stem cell transplantation (SCT) have become an effective care for many patients with hematological malignancies. Harvesting the stem cells is one the most important parts of SCT. The two most commonly used mobilization regimens are the use of granulocyte colony-stimulating factor (G-CSF) or G-CSF plus chemotherapy. However, about 10-30% of patients are unab...

(1) Background: There are no high-throughput microtissue platforms for generating bone marrow micro-ossicles. Herein, we describe a method the assembly of arrays microtissues from stromal cells (BMSC) in vitro and their maturation into micro-ossicles vivo. (2) Methods: Discs with 50 microwells were used to assemble 3 × 105 BMSCs each on nylon mesh carrier. Microtissues cultured chondrogenic ind...

in this study granulocyte-macrophage colony stimulating factor (csf) was prepared from rabbit and guinea pig lung tissues. the biological activity of these csfs was measured on semisolid agar culture and compared with those of mouse and rat. weight of animals, incubation time and cross-reactivity of csfs with different bone marrow sources were also investigated. the results showed that both rab...

The medullary niche is a complex ecosystem that essential to maintain homeostasis for resident cells. Indeed, the bone marrow, which includes extracellular matrix and various cell types, such as mesenchymal stem cells, osteoblasts, endothelial deeply involved in hematopoietic regulation through direct cell-cell interactions, well cytokine production. To closely mimic this vivo structure conduct...

Background: Protection of hematopoietic system has become a primary goal in the development of novel medical countermeasures against ionization radiation and radiotherapy. This study was to explore the role of rapamycin in normal tissues against radiation. Materials and Methods: Mice were pretreated with rapamycin by i.p. every other day for five times before 5 Gy or 8.5 Gy γ-ray whole bo...