Cultural characteristics and serological relationships of mycoplasmas from the bovine vagina, mastitic milk, and other sources were studied. Parasitic strains could be distinguished from saprophytic strains in that they required incorporation of horse serum into the PPLO agar for growth, produced surface crystallization on PPLO agar and did not ferment carbohydrates. All strains were haemolytic...

Background and Aim Mastitis is one of the most vital noteworthy monetary risks to dairy ranchers and affects reproductive performance in dairy cattle. However, subclinical mastitis (SCM) negatively affects milk quality and quantity and associated with economic losses as clinical mastitis. It is recognizable only by additional testing. Somatic cell count (SCC) is currently used worldwide for the...

Discrimination of Staphylococcus aureus strains, isolated from camel abscesses and mastitic milk of camel, cattle and goats, on the basis of 16S-23S ribosomal RNA intergenic space polymorphism was carried out. Two sets of primers were used for amplification of DNA of intergenic space; the one having a highly conserved sequence in eubacterial 23S rRNA transcript, while the other having less cons...

Determining the species of mycoplasma isolated from culture-positive milk samples is important for understanding clinical significance their detection. Between August 2016 and December 2019, 214,518 2,757 dairy herds were submitted to Quality Milk Production Services (QMPS) at Cornell University culture. From these samples, 3,728 collected 204 culture positive. Based on request herd managers, o...

Twenty one staphylococcus aureus isolates of bovine mastitic milk origin from herds at different locations were analysed for their coa gene products and RFLP patterns. One of the isolates was coa gene deficient whereas the rest revealed polymorphism in the coa gene. The isolates revealed three different types of coa gene products (600, 680 or 850 bp) and three distinct RFLP patterns were obtain...

Mastitis is a major dairy herd problem mainly caused by Staphylococcus aureus. The Staphylococcus aureus isolated strains from mastitic milk samples were subjected to molecular characterization of coa gene and phylogenetic tree (using MEGA6.1 software package) was constructed which revealed that the coa gene of S. aureus strains, could be grouped in two clades which were closely related to S. a...

AIM This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt. MATERIALS AND METHODS A total of 81 milk samples from 46 normal buffalo milk samples and 35 SCM buffalo milk samples at private dairy farms of Egypt were used in this study. CNS were identified using phenotyp...