نتایج جستجو برای: membrane crystallization
تعداد نتایج: 411323 فیلتر نتایج به سال:
A general strategy for the expression of bacterial membrane transport and receptor genes in Escherichia coli is described. Expression is amplified so that the encoded proteins comprise 5-35% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGSH6 or a Strep tag at the C-terminus. These enable purification in mg quantities for crystallization and NMR st...
The primary act of the adhesion-condensation mechanism underlying membrane fusion is considered. This act involves the formation of a close dehydrated contact between membranes and the subsequent crystallization of molecules of the external monolayers in the contact region. Crystallization associated with a decrease in the area per molecule gives rise to elastic stresses which cause a disruptio...
Since the early days of structural biology, membrane proteins have kept the attention of scientists. However, due to their natural environment, their surfaces highlight dual properties, partly hydrophobic but also partly hydrophilic, making these proteins impossible to handle in aqueous solutions. In the 1980s new concepts for the purification of such proteins based on the use of detergents eme...
The axial development of calcium sulfate dihydrate (gypsum) scaling on selected reverse osmosis (RO) membrane surfaces was investigated experimentally in a plate-and-frame RO system. Scaling experiments with model solutions demonstrated progressive axial development of surface gypsum crystals along the membrane surface. The impact of surface crystallization was characterized via flux decline me...
This review covers new experimental and theoretical physical research related to the formation of polymeric membranes by phase separation of a polymer solution, and to the morphology of these membranes. Two main phase separation processes for polymeric membrane formation are discussed: thermally induced phase separation and immersion precipitation. Special attention is paid to phase transitions...
Co-crystallization of membrane proteins with antibody fragments may emerge as a general tool to facilitate crystal growth and improve crystal quality. The bound antibody fragment enlarges the hydrophilic part of the mostly hydrophobic membrane protein, thereby increasing the interaction area for possible protein-protein contacts in the crystal. Additionally, it may restrain flexible parts or lo...
BACKGROUND Reactive oxygen species play important roles in renal calcium crystallization. In this study, we examined the effects of catechin, which have been shown to have antioxidant properties on the renal calcium crystallization. METHODS In the vitro experiment, the changes of the mitochondrial membrane potential, expression of superoxide dismutase (SOD), 4-hydroxynonenal (4-HNE), cytochro...
We report a numerical simulation of the phase diagram of a simple model for membrane proteins constrained to move in a plane. In analogy with the corresponding three dimensional models, the liquid-gas transition becomes metastable as the range of attraction decreases. Spontaneous crystallization happens much more readily in the two dimensional models rather than in their three dimensional count...
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