The purpose of this work is to employ a three-dimensional model examine breast cancer metastasis from various perspectives. Using solid tumor exposed standard chemotherapeutic treatment scheme, we aim replicate the earliest stages metastatic cascade, focusing on drug's effect invasive cells.

Background: Intra-operative molecular diagnostic assays are currently used for the detection of lymph node metastases. The objective of this study was to find new biomarkers to improve diagnostic accuracy in the detection of metastatic axillary lymph nodes in breast cancer patients. Methods: We applied an absolute quantitative real-time RT-PCR to quantitate the expression of CK19, KLK11, and CL...

Rapid improvements in the detection and tracking of early-stage tumor progression aim to guide decisions regarding cancer treatments as well as predict metastatic recurrence in patients following surgery. Mathematical models may have the potential to further assist in estimating metastatic risk, particularly when paired with in vivo tumor data that faithfully represent all stages of disease pro...

This study reports an easily accomplished and reliable model of metastatic tumor in the brains of mice. Five experimental groups of female C3H/Bi mice received left intracardiac injections of a syngeneic KHT sarcoma cell suspension (1 X 10(5) cells) and were followed until death. Two groups of mice also received 3,000 rads of radiation to a limited cardiac port 24 hr after tumor injections. All...

Viola odorata as a medical herb is used in liver disorders and relieving cancer pain. In the present study, the cytotoxic, antioxidant, and anti-metastatic properties of Viola odorata hydro-alcoholic extract (VOE) were investigated in 4T1 breast cancer model. After treatment of 4T1 breast cancer cells with VOE, cell viability was measured by MTT assay. The implanted mice were treated with diffe...

Viola odorata as a medical herb is used in liver disorders and relieving cancer pain. In the present study, the cytotoxic, antioxidant, and anti-metastatic properties of Viola odorata hydro-alcoholic extract (VOE) were investigated in 4T1 breast cancer model. After treatment of 4T1 breast cancer cells with VOE, cell viability was measured by MTT assay. The implanted mice were treated with diffe...

AIM: Colon cancer is one of the main health problems worldwide. Cancer stem cells (CSCs) are referred to as tumor-initiating involved in tumor heterogeneity and dormancy. CSCs can cause drug resistance, metastasis, recurrence primary metastatic cancers. The interactions survival trends colon with other may be an alternative route for effective treatment. In our study, we aimed evaluate effects ...

Colorectal cancer (CRC) cells are traditionally considered unresponsive to TGF? due mutations in the receptors and/or downstream signaling molecules. influences CRC only indirectly via stromal cells, such as cancer-associated fibroblasts. However, cell ability directly respond currently remains unexplored. This represents a missed opportunity for diagnostic and therapeutic interventions. Method...

The present study aimed to investigate the roles of the vascular endothelial cell growth factor (VEGF) and micro (mi)RNA-210 in the metastasis of primary medulloblastoma (MB) tumors. A total of 86 adult patients diagnosed with cerebellar MB were enrolled in the present study, of which 11 patients had metastatic MB in the subarachnoid space. The following samples were collected: MB primary tumor...

Metastatic tumors have been shown to establish microenvironments in distant tissues that are permissive to disseminated tumor cells. Hematopoietic cells contribute to this microenvironment, yet the precise initiating events responsible for establishing the pre-metastatic niche remain unclear. Here, we tracked the developmental fate of hematopoietic stem and progenitor cells (HSPC) in tumor-bear...