Biopharmaceuticals are an integral part of modern medicine and pharmacy. Both, the development and the biotechnological production of biopharmaceuticals are highly cost-intensive and require suitable expression systems. In this review we discuss established and emerging tools for reengineering the methylotrophic yeast Pichia pastoris for biopharmaceutical production. Recent advancements of this...

Background: Methanol is regarded as a biorenewable platform feedstock because nearly all bioresources can be converted into methanol through syngas. Biological conversion of methanol using synthetic methylotrophs has thus gained worldwide attention. Results: Herein, to endow Escherichia coli with the ability to utilize methanol, an artificial linear methanol assimilation pathway was assembled i...

abstract: the commercial development of food products originated from the plants as sources of antioxidants to increase the health benefit and stabilize the food is of current interest. this study was aimed at obtaining dill extract using cold solvent method by three solvents, (methanol, acetone, and hexane) and examining the antioxidant potency and determining the oxidative stability of sunflo...

The Escherichia coli phytase gene appA was highly expressed in the methylotrophic yeast Pichia pastoris under the control of the AOX1 promoter. Replacement of culture medium with fresh medium in order to remove repressing glycerol and metabolic wastes prior to methanol induction significantly improved phytase expression. The phytase activity level was enhanced from 118 to 204 U/ml at the flask ...

The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxidation to CO2 surpassed methanol assimilation and methanogenesis from CO2/H2 a...

salvia chorassanica bunge is one of the iranian endemic species of salvia. there is not any reported literature on s. chorassanica. this study was designed to examine the in-vitro anti-proliferative and proapoptotic effects of the methanol extract of s. chorassanica and its fractions on hela cell line. cells were cultured in ex-cell®, an animal free medium specially designed for hela cell line ...

Abstract We used the methanol-to-hydrocarbon (MTH) reaction as a shape-selective model to investigate coke formation in zeolite H-Ferrierite. Despite being 2D topology terms of channel propagation, FER framework displays lattice expansion all three dimensions space upon deactivation. Therefore, volume unit cell is an excellent X-Ray diffraction (XRD) descriptor for catalyst A with dummy atoms a...

effect of methanol concentration and channel depth of bipolar plates on the performance of single direct methanol fuel cell was investigated experimentally. the membrane used in this experiment was nafion117. dimension of the single cell were 10cm 10 cm. methanol concentrations of 0.50, 1.0, 1.5, 2.0, 3.0 molar were used. channel depth of the bipolar plates were chosen to be 1.0, 1.5, 2.0 mm, a...