Pseudorabies virus was photoinactivated with a combination of methylene blue dye, light, and electricity. Viral suspensions were mixed with variable amounts of methylene blue dye and then were placed in a current source apparatus. Total inactivation of pseudorabies virus (1.7 X 10(6) 50% tissue culture infective doses per ml) was achieved with constant mixing, a methylene blue dye concentration...

The MoFe protein from Azotobacter vinelandii catalyzes the reduction of methylene blue and other oxidants by H2 under anaerobic conditions. H2 uptake followed manometrically or by 3H2 transfer from the gas to aqueous phase occurs concomitantly with methylene blue disappearance monitored optically or coulometrically. The stoichiometry was found to be 1:1 methylene blue/H2. MoFe protein oxidized ...

OBJECTIVE Seromas or lymphoceles are common postoperative complications. This series presents 3 patients with lower extremity seromas refractory to treatment that required lymphatic mapping and lymphatic ligation for closure, and in 1 case, diagnosis. METHODS Lymphatic mapping procedure consisted of intraoperative injection of subcutaneous tissue with methylene blue distal to the seroma with ...

background and objectives : due to the extensive use of antibiotics, the spread of drug-resistant bacteria is one of the most worrisome threats to public health. one strategy that can be used to overcome potential shortcomings might be the inactivation of these organisms by photodynamic therapy. in this study, we have investigated whether drug-resistant wound-associated organisms (staphylococcu...

abdomen of a DIO mouse. Peaks are labeled as in ref.[2]. Fig.2 : IZQC spectrum of extract BAT (red) and WAT (blue) tissue. The methylene/water iZQC peak (P2/Water) is characteristic of the only BAT tissue. Fig. 3: Comparison of the in vivo iZQC spectra from a 8 weeks old C57 mouse (red) and a 2 years old ob/ob mouse (blue). The methylene/water peak (arrow) is considerably reduced in the obese m...