The accessibility of histone H5 in chromatin was examined with monoclonal antibodies recognizing several epitopes of the globular region (GH5) of the histone (Rózalski, M., Lafleur, L., and Ruiz-Carrillo, A. (1985) J. Biol. Chem. 260, 14379-14385). The stoichiometry of the chromatin-antibody complexes indicated that while 0-86% of the H5 molecules were able to react, depending on the particular...

Calcium release from sarcoplasmic reticulum vesicles presumably derived from longitudinal tubules (LSR) and terminal cisternae (HSR) of rabbit skeletal muscle was investigated by dual wavelength spectrophotometry using the calcium-indicator antipyrylazo III. In 120 mM KCl, 5 mM MgCl2, 30 microM, CaCl2, 50 microM MgATP, 100 microM antipyrylazo III, 40 mM histidine (pH 6.8, 25 degrees C), LSR and...

We constructed SP6-human beta-globin derivative plasmids that included possible donor site (5' splice site) sequences at a specified position within the first intron. The runoff transcripts from these templates truncated in the second exon were examined for splicing in a nuclear extract from HeLa cells. In addition to the products from the authentic donor site, a corresponding set of novel prod...

1. Honey bees require minerals for a complete diet. However, from flowers can be inadequate in concentration and composition. Therefore, honey may drink ‘dirty water’ natural sources such as puddles. Some research has attempted to simulate this through bee bioassays, but date, these have tested individually, not mixtures would occur nature. Here, the first time, we use bioassays which range of ...

An effective combination of oxidation medium, ball milling parameters, and copper additive disperstiy ensuring fast aluminum scrap reaction with high hydrogen yield, was suggested. Different parameters (5, 10, 15 mm steel balls; 1 2 h; unidirectional bidirectional rotation modes) were tested for Al-10 wt.% Cu (50–70 μm) composition. The samples milled 5 (2 h) 10 (1 balls contained undesirable i...

A dramatic reduction in man-made CO2 emissions could be achieved if the cost of electricity generated from concentrated solar power (CSP) plants become competitive with fossil-fuel-derived electricity. The heat-to-electricity conversion efficiency CSP may significantly increased (and associated decreased) by operating turbines inlet temperatures ≥750 °C instead ≤550 °C, and using thermal energy...