The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle i...

Two simple enzyme immunoassays, a conventional microplate and dot-ELISA, were developed to detect specific IgM antibodies using pool sonicated antigen prepared from three of the most reactive serovars of Leptospira associated with disease in Thailand. Both assays were evaluated and compared with the standard microscopic agglutination test (MAT) performed with 343 serum samples. A battery of 16 ...

BACKGROUND Korea is considered an iodine sufficient country, and several studies have been conducted regarding iodine status in healthy Korean adults, pregnant women, and preschool children. However, data on iodine status in Korean school-age children are lacking. Therefore, the iodine nutrition status of Korean school-age children was investigated by measuring urine iodine concentration (UIC)....

Deep-sea hydrothermal vent species are widely dispersed among habitat islands found along the global mid-ocean ridge system. We examine factors that affect population structure, gene flow and isolation in vent-endemic mussels of the genus Bathymodiolus from the eastern Pacific Ocean. Mussels were sampled from localities including the Galapagos Rift (GAR, 0 degrees 48' N; 86 degrees 10' W) and t...

In vitro experiments in plastic receptacles are the basis of characterization of new photosensitizers (PSs) for the photodynamic therapy. We recently reported that lipophilic PSs adhere to cell culture microplates in a kinetic-like manner (Engelhardt et al., 2011). In the current study, we examined the interaction and phototoxic effects of the microplate-adhered PS in cancer cells. Therefore, w...

A new optical sensing microplate was developed for rapid screening for the presence of biogenic amines (BAs) in seafood samples with high sensitivity. The deposition of a sensing spot (containing a chameleon dye (Py-1) in a polymeric cocktail) on the bottom of the wells of a standard microplate renders the plate a new sensing tool for a rapid and parallel detection of up to 96 (real) samples. T...

In the Katha Range of central Myanmar, lithologic tracers and pressure-temperature-deformation-time data identify Cambro-Ordovician, Indian-affinity Tethyan Himalaya Series, located ∼700 km from their easternmost outcrop in S-Tibet, ∼450 Himalayan rocks Eastern Syntaxis. Metamorphism began at ∼65 Ma, peaked ∼45 Ma (∼510°C, 0.93 GPa), exhumation/cooling (∼25°C/Myr) occurred until ∼30 a subductio...

To evaluate the effect of eucalyptus, sappan, and cinnamon leaf bark extract on percentage S. mutans biofilm formation. The test group was divided into a negative control (1% Dimethyl sulfoxide), treatment (eucalyptus, leaves at concentrations 50%, 25%, 12.5%, respectively), positive (0.2% chlorhexidine). Each concentration barks extract, Brain Heart Infusion Broth (BHI-B), bacteria according t...