MicroRNA profiling: approaches and considerations. Cheng2, 3 and Muneesh Tewari2, 3, 4, 5. Abstract MicroRNAs miRNAs are.Nat Rev Genet. Pritchard CC1, Cheng HH, Tewari M. currently available microRNA microarrays in profiling pre-microRNA. H.H. MicroRNA profiling: Approaches and considerations.MicroRNA profiling: approaches and considerations. Selected Emerging Technologies for miRNA Profiling. ...

Purpose: To investigate the effect of microRNA-16 (miR-16) on glioma cell migration and invasiveness, mechanism involved.Methods: MicroRNA-16 mimic or inhibitor was transfected into human (SHG44) cells. Cell migration, invasiveness morphology were determined using scratch test, Transwell invasion assay, immunohistochemical staining, respectively. Expressions bcl-2, MMP-9 MMP-2, NF-κB1 proteins ...

Accumulating evidence indicates that numerous microRNAs are involved in the tumorigenesis and progression of gastric cancer, while the clinical significance of microRNA-214 in gastric cancer is poorly understood and the exact role of microRNA-214 in gastric cancer remains obscure. In the present study, expression levels of microRNA-214 in 80 gastric carcinoma tissues, 18 nontumourous gastric ti...

MicroRNAs have been appreciated in various cellular functions, including the regulation of angiogenesis. Mesenchymal-stem-cells (MSCs) transplanted to the MI heart improve cardiac function through paracrine-mediated angiogenesis. However, whether microRNAs regulate MSC induced angiogenesis remains to be clarified. Using microRNA microarray analysis, we identified a microRNA expression profile i...

background: microrna is a type of small rna of about 22 nucleotide length. most popular mirnas are found in the liver and are extensively important in determining the biological and clinical functions. microrna, in host cells, may impact the replication of viruses either positively or negatively. in this study the mir-122 expression was examined and compared in three groups, two sample groups o...

micrornas are tiny, 18-25 nucleotides in length, non-coding rna molecules preserved throughout evolution. these molecules primarily control gene expression at the post-transcriptional and transcriptional levels. micrornas control target gene expression by a phenomenon known as rna interference. rna interference based therapeutics that utilize target gene silencing/degradation by specific micror...

RNA processing is essential factor for synthesis of functional and structural proteins in eukaryote cells. In eukaryote organisms it will be initiated with transcription of DNA in nucleolus and terminated to mRNA translation in cytoplasm, finally mRNA degraded. Protein synthesis followed as different steps, includes 5' capping, poly adenylating, processing and transferring from nucleolus to cyt...

Gene duplication provides large numbers of new genes that can lead to the evolution of new functions. Duplicated genes can diverge by changes in sequences, expression patterns, and functions. MicroRNAs play an important role in the regulation of gene expression in many eukaryotes. After duplication, two paralogs may diverge in their microRNA binding sites, which might impact their expression an...

The rapid increase and poor survival of esophageal adenocarcinoma (EAC) have led to significant efforts to promote early detection. Given that the premalignant lesion of Barrett's esophagus (BE) is the major known risk factor for EAC, multiple investigators have studied biomarker signatures that can predict malignant progression of BE to EAC. MicroRNAs, a novel class of gene regulators, are sma...

BACKGROUND The prognosis of non-small-cell lung cancer (NSCLC) is poor yet mechanistic understanding and therapeutic options remain limited. We investigated the biological and clinical significance of microRNA-130b and its relationship with apoptosis in NSCLC. METHODS The level of microRNA-130b in relationship with the expression of PPARγ, VEGF-A, BCL-2 and apoptosis were analyzed in 91 lung ...