In order to increase the yield of haploid cucumber plants obtained from in vitro culture of unpollinated ovules, anther and microspore, we have studied several factors: the nature and concentration of plant growth regulators, the genotypes, the flower bud lengths, and the stages of microspore development. Among the different growth regulators tested, thidiazuron (TDZ) was the best for gynogenes...

Potassium antimonite was used to locate calcium in the anthers of Torenia fournieri (Linderniaceae). Abundant calcium precipitates accumulate in the microsporocyte cytoplasm. After meiosis, calcium precipitates are abundant on the microspore wall, as well as the callosic wall of each tetraspore. A large number of calcium precipitates also occur on the outer membranes of the tapetal cells, and i...

Members of the glucan synthase-like (GSL) family are believed to be involved in synthesis of the cell-wall component callose in specialized locations throughout the plant. We identified two members of the Arabidopsis GSL gene family, GSL8 and GSL10, that are independently required for male gametophyte development and plant growth. Analysis of gsl8 and gsl10 mutant pollen during development reve...

Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products....

To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would signif...

COMPARISON of microsporogenesis and microspore development A in a triploid Tradescantia has shown significant deviations from the normal diploid behavior. Occasional dicentric chromatids a t meiosis are found in diploid Tradescantias, but inversion crossovers in the triploid are much more frequent. An analysis of microspore development in a triploid Tradescantia shows that chromosome number has...

In plant breeding studies, dihaploidization method is used to obtain homozygous pure lines develop varieties in a shorter time. Dihaploidization applied most types of vegetables such as wheat and corn, well vegetable species. Androgenesis methods (anther microspore culture) gynogenesis parthenogenesis (ovula ovary are haploid plants. One the androgenetic methods, shed-microspore culture has bee...

androgenesis via anther culture or microspore culture is one of the current methods for producing haploid and double haploid plants in maize. to use of this method in maize breeding program it should be able to regenerate enough plantlets. anthers culture usually is carried out indirectly via callus induction and regeneration on at least two different media. in this study a responsive genotype,...