Doubled haploidy methodology is commonly used in many agronomically important crops to speed the development of new cultivars. There are a number of advantages in using haploidy technology in both practical application (varietal development, mutagenesis, transformation) and basic research (genomics, biochemical, and physiological studies). Haploid plants are commonly produced using one of four ...

The Arabidopsis sporophytic tapetum undergoes a programmed degeneration process to secrete lipid and other materials to support pollen development. However, the molecular mechanism regulating the degeneration process is unknown. To gain insight into this molecular mechanism, we first determined that the most critical period for tapetal secretion to support pollen development is from the vacuola...

The present investigation deals with morphological and embryological studies of Rhynchelytrum repens (Willd) C.E. Hubbard. The development of anther walls are found to be Monocotyledonous type. The tapetal cells are substantially large, glandular and uninucleate. The middle layer is ephemeral and their cells are small in size. It is sandwiched between endothecial and tapetal layer. The endothec...

To identify factors that are required for proper pollen wall formation, we have characterized the T-DNA-tagged, dex1 mutation of Arabidopsis, which results in defective pollen wall pattern formation. This study reports the isolation and molecular characterization of DEX1 and morphological and ultrastructural analyses of dex1 plants. DEX1 encodes a novel plant protein that is predicted to be mem...