Six ruminally cannulated Holstein steers (initial BW = 189 +/- 11 kg) housed in metabolism crates were used in a 6 x 6 Latin square to study effects of ruminal ammonia load on Leu utilization. All steers received a diet based on soybean hulls (2.7 kg of DM/d), ruminal infusions of 200 g of acetate/d, 200 g of propionate/d, and 50 g of butyrate/d, as well as an abomasal infusion of 300 g of gluc...

Tea waste (TW) has a protein content of approximately 30% and the potential to replace other sources. However, effect dietary TW supplement is still ambiguous. The objective this study was investigate three levels on performances physiological changes in goats. Nine multiparous crossbred Saanen lactating goats were arranged replicates 3 × Latin square. groups fed with 0, 5, or 10 by weight [wt%...

Generally, <30% of dairy cattle's nitrogen intake is retained in milk. Large amounts of nitrogen are excreted in manure, especially in urine, with damaging impacts on the environment. This study explores the effect of lowering dietary degradable nitrogen supplies--while maintaining metabolisable protein--on dairy cows' performance, nitrogen use efficiency and gas emissions (NH3, N2O, CH4) at ba...

Eight lactating cows were fed 4 diets in which dietary crude protein (CP) was increased steps of approximately 2 percentage units from 11 to 17% DM by replacing high-moisture corn with soybean meal supplemented rumen-protected Met maintain a Lys:Met ratio 3:1 metabolizable protein. Trial design replicated × Latin square; experimental periods lasted 28 d, data and sample collection being perform...

Four ruminally cannulated Holstein cows were used in a 4 × 4 Latin square design to determine the effect of feeding linted, delinted and extruded linted whole cottonseed (WCS) on nutrient digestibility and ruminal fermentation. Result showed that crude protein digestibility was lower in linted and delinted WCS treatments than control and extruded linted WCS treatment. Cows fed extruded delinted...

Increased availability of rapidly fermentable carbohydrates and a great proportion of corn-derived CP in the diet may result in a degradable intake protein (DIP) deficit. Therefore, ruminal DIP deficit may result from high dietary inclusion of processed corn grain and small to moderate inclusion of corn distillers grains (DG). Two experiments were conducted to evaluate the effect of increasing ...

Nitrogen (N) appears to be a limiting dietary resource for elasmobranchs, required not only for protein growth but also for urea-based osmoregulation. Building on recent evidence that the toxicant ammonia can be taken up actively at the gills of the shark and made into the valuable osmolyte urea, we demonstrate that the uptake exhibits classic Michaelis-Menten saturation kinetics with an affini...

Ruminal ciliates have been grown in continuous culture in chemically defined media and in the absence of viable bacteria. Oligotrichic ruminal ciliates seem to require insoluble carbohydrates for growth; the holotrichic ciliates require soluble carbohydrates, but at low concentrations. Both groups of ciliates utilize amino acids as their principal nitrogen source when these are supplied in micr...

................................................................................................................................ ii ACKNOWLEDGEMENTS ........................................................................................................ iv TABLE OF CONTENTS ............................................................................................................. v LIST OF TA...