A cytological study was made of short term primary cultures of monkey kidney epithelial cells grown in serum-containing (M-H) and synthetic (SM-2) media. The mitotic activity of the cultures reached a peak 3 to 5 days after seeding, and rapidly declined if the medium was not changed. With replenishment at 48 hour intervals a high mitotic rate was maintained through the 8th day. Cultures subject...

It has been shown that 2-methoxyestradiol (2-ME) inhibits cell proliferation and DNA synthesis in human aortic smooth muscle cells. However, the cellular mechanisms underlying the antiproliferative activity of 2-ME are unclear. The present study was performed to explore the cellular mechanisms whereby 2-ME leads to growth inhibition and apoptosis of human smooth muscle cells. Our results demons...

Young adult male rats received 1,2-dimethylhydrazine (25 mg/kg) twice weekly for 2 months and once a week thereafter for up to 6 months. Histological samples of duodenum, jejunum, and upper, mid-, and terminal ileum were prepared from groups killed at each month. Using cell counts, the average number of epithelial cells was determined per representative section of villi and crypts and was used ...

Aim: To evaluate the premalignant and malignant lesions of the uterine cervix on light microscopy for apoptosis. To calculate and correlate the apoptotic index (AI), mitotic index (MI) and turnover index of the lesions. Materials and methods: A 2-year retrospective (November 2007 to October 2009) and 1-year prospective study (November 2009 to October 2010). A total of 95 cases of premalignant a...

A series of derivatives of 5,6-diphenylpyridazin-3-one (DPP) was examined for interactions with calf brain tubulin following the demonstration that many members of the class caused significant mitotic effects in intact animals, while others had activity against murine P388 leukemia. In L1210 cells several DPP derivatives caused a rise in the mitotic index which correlated well with the cytotoxi...

PURPOSE This work aimed to investigate the effect of costunolide, a sesquiterpene lactone isolated from Michelia compressa, on cell cycle distribution and radiosensitivity of human hepatocellular carcinoma (HCC) cells. METHODS The assessment used in this study included: cell viability assay, cell cycle analysis by DNA histogram, expression of phosphorylated histone H3 (Ser 10) by flow cytomet...

The effects of colchicine and heavy water on cell division in wheat roots have been examined by means of mitotic index measurements correlated with an electron-microscope examination of the tissue. Heavy water alone caused an arrest of mitosis both in normal tissue and in roots which had been brought into partial synchrony of division by means of 5-aminouracil. Colchicine alone caused an accumu...

The current model for endocrine cell specification in the pancreas invokes high-level production of the transcription factor Neurogenin 3 (Neurog3) in Sox9(+) bipotent epithelial cells as the trigger for endocrine commitment, cell cycle exit, and rapid delamination toward proto-islet clusters. This model posits a transient Neurog3 expression state and short epithelial residence period. We show,...

In the present study, the division modalities and regularity of rat cardiomyocytes from different developing stages were investigated with immunohistochemistry, transmission electron microscopy and cell culture in vitro. The results showed that the cells with mitotic figure and binuclear cells exist in the normal cardiomyocytes in prenatal and postnatal life. There are two types of binuclear ca...

BACKGROUND The histopathologic distinction between typical carcinoid (TC) and atypical carcinoid (AC) of the lung is based largely on mitotic index. Ki-67 may aid in separation of these tumors, as well as the distinction from large cell neuroendocrine carcinoma (LCNEC). METHODS We identified 55 surgically resected primary neuroendocrine lung tumors (39 TC, 7 AC, 9 LCNEC) based on mitotic rate...