Abstract Thermophilic Argonaute proteins (Agos) have been shown to utilize small DNA guides for cleaving complementary in vitro, which shows great potential nucleic acid detection. In this study, we explored mesophilic Agos the detection of molecule by cooperating with allosteric transcription factors (aTFs). Two from bacteria, Paenibacillus borealis ( Pb Ago) and Brevibacillus laterosporus Bl ...

Granules from a mesophilic upflow anaerobic sludge blanket reactor were disintegrated, and bacteria utilizing only hydrogen or formate or both hydrogen and formate were added to investigate the role of interspecies electron transfer during degradation of propionate and butyrate. The data indicate that the major electron transfer occurred via interspecies hydrogen transfer, while interspecies fo...

Roth, Norman G. (Whirlpool Corp., St. Joseph, Mich.) and Robert B. Wheaton. Continuity of psychrophilic and mesophilic growth characteristics in the genus Arthrobacter. J. Bacteriol. 83:551-555. 1962.-The effect of temperature on growth of seven members of the genus Arthrobacter was determined at 0, 7, 20, 30, and 37 C. In general, no sharp cutoff point was observed between growth-temperature r...

Studies with a diversity of hyperthermophilic and mesophilic dissimilatory Fe(III)-reducing Bacteria and Archaea demonstrated that some of these organisms are capable of precipitating gold by reducing Au(III) to Au(0) with hydrogen as the electron donor. These studies suggest that models for the formation of gold deposits in both hydrothermal and cooler environments should consider the possibil...

Composting is the process of decomposition and transformation solid organic substances by microorganisms (mainly bacteria fungi) into a stable material, which can be used (depending on characteristics) in agriculture, instead chemical fertilizers or land improvement works (soil improvement. The composting consists two stages: mechanical treatment (fermentation). In case treatment, waste crushed...

Sushi, a very popular food worldwide became a popular ready-to-eat food selling in supermarkets, but it exhibit distinct features, which are associated with microbiological hazards. Therefore, MAP technology, known to reduce aerobic bacteria in fishery products, was used to improve quality of ready-to-eat salmon sushi in this study. Salmon sushi were packaged with air (control), 50%N2 /50%CO2 (...

The presence of bacteria and fungi in indoor air pose a serious problem from the point of view of health protection and environmental engineering. Precise determination of various groups of microorganisms indoors is necessary both to estimate the health hazard and to create standards for indoor air quality control. This is especially important in such densely populated facilities like education...

Work on the relationship between hyperthermophile protein dynamics, stability and activity is reviewed. Neutron spectroscopy has been applied to measure and compare the macromolecular dynamics of various hyperthermophilic and mesophilic proteins, under different conditions. First, molecular dynamics have been analyzed for the hyperthermophile malate dehydrogenase from Methanococcus jannaschii a...