1Departments of Gynecology and Obstetrics and Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA 2Department of Pathology, Norwegian Radium Hospital, Rikshospitalet University Hospital, Montebello, 0310 Oslo, Norway 3Departments of Pathology and Microbiology and Immunology, The University of South Carolina School of Medicine, Columbia, SC 29203, USA 4Department of M...

In the era of Human Immunodeficiency virus, emerging and re-emerging of various microbial infections, the status of a clinical microbiology laboratory performance is different in the developed and developing nations. On the one side application of rapid, accurate and advanced molecular laboratory techniques have contributed to better diagnosis, patient care and management in developed nations, ...

background: group b streptococcus (gbs) is the major cause of serious life threatening infections in neonates, pregnant women, and other adults with underlying diseases. capsular polysaccharide typing is a significant way for epidemiological studies of gbs, the pathogenesis, and other studies associated with gbs infections including surveillance programs and vaccine development in future. molec...

OBJECTIVE Fast detection of β-lactamase (bla) genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited bla gene types, these methods have significant limitations, such as their failure to detect almost all clinically available bla genes. We have evaluated a further refinement of our fast and accurate molecular me...

Background: Some antigens of H. pylori are excreted into the stool of infected people. These antigens can be used to detect the infection by immunoassays such as ELISA. Our aim was to identify these antigens by immunoblotting and affinity chromatography techniques. Methods: Four different antigenic preparations, namely, whole cell sonicate (WCS), outer membrane proteins (OMPs), cytoplasmic anti...

mycobacterium tuberculosis (h37rv strain) was used in this study. the bacterial cells were disintegrated by sonication. the separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using sds and 2me. eight protein molecules with molecular weights ranging from 6.3 ...

introduction: screening of patients with anterior abdominal penetrating trauma in need for laparotomy is an important issue in management of these cases. this study aimed to compare the accuracy of abdominal wall ultrasonography (awu) and local wound exploration (lwe) in this regard. methods: this diagnostic accuracy study was conducted on ≥ 18 year-old patients presenting to emergency departme...