A murine monoclonal antibody (MAb) which binds to human metastatic gastrointestinal adenocarcinomas can be adminis tered safely and has tumor effects in some patients. Its thera peutic effect was assessed in 20 patients with measurable advanced colorectal carcinoma that was refractory to prior sur gical resection, chemotherapy, and/or radiotherapy. All patients had agreed to receive no other th...

This report investigates the application of monoclonal antibody A7 and its drug conjugate in locally controlling colorectal cancer. The experimental protocol consisted of local retention, lymphatic delivery, normal organ distribution, systemic toxicity, and tumoricidal effects. When 125I-labeled monoclonal antibody (Mab) A7 was injected into the pelvis and the thigh of Balb/c mice, a high local...

Chromium is one of main pollution in sea water, and the establishment detecting kit research hot topic. In this study, immunized mice were obtained by immunogen Cr 3+ -EDTA-BSA, hybridoma strains that secrete -EDTA monoclonal antibody selected. mAb prepared with vivo method. Then, Cr-kit for measurement chromium was developed, its application executed water seafood. Two A321 A424 screened. The ...

Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was...

An immunotoxin conjugate of a murine monoclonal antibody against human transferrin receptors and the A chain of ricin was examined for its potential to inhibit selectively the growth of retinal pigment epithelial (RPE) cells which grow in an uncontrolled manner in proliferative vitreoretinopathy. The probable efficacy of such an agent in vivo stems from the observation that actively proliferati...

Background: Allergy to Saffron (Crocus sativus) pollen has been described in people involved in processing of saffron flower stamens. Profilins have been identified as a pan-allergen in different plant pollens and foods. This molecule is an actin-binding pro-tein with a molecular weight of 12-16 kDa found in eukaryotic species. Objective: The aim of this study was to generate monoclonal antibod...

Background: Monoclonal antibodies have confirmed their merit as biotherapeutics across a wide spectrum of diseases, including cancer, heart disease, infection, and immune disorders. Materials and Methods: The dynamics of 89Zr-labelled monoclonal antibodies (MAb) after injection into the human body are modelled. This modified biokinetic model can be used for dose assessment not only for 89Zr-lab...

Abstract Complement dependent cytotoxicity (CDC) is a key Fc mediated function of monoclonal antibody (mAb) therapeutics. mAb binding to antigen triggers complex molecular cascade with sequential recruitment serum proteins, eventuating in target cell lysis. Here we demonstrate CDC quantification streamlined, vitro, advanced flow cytometry assay. Anti-CD20 mAbs were incubated lines 96- or 384-we...

Chinese hamster ovary (CHO) cells are the leading mammalian cell expression platform for biotherapeutic recombinant molecules yet some proteins remain difficult to express (DTE) in this, and other, systems. In lines expressing DTE proteins, cellular processes restore proteostasis can be triggered when folding modification capabilities exceeded, including unfolded protein response ER-associated ...

We have generated the transgenic Tabaco plants expressing multiple monoclonal antibody (mAb) CO7-1A × BR55 by cross-pollinating with mAb CO17-1A and mAb BR55. We have demonstrated the anti-cancer effect of plant-derived multiple mAb CO17-1A × BR55. We find that co-treatment of colorectal mAbs (anti-epithelial cellular adhesion molecule (EpCAM), plant-derived monoclonal antibody (mAb(P)) CO17-1A...