Pelvic tuberculosis is an infectious disease caused by the bacterium Mycobacterium tuberculosis (MTB) (95%) or Mycobacterium bovis (5%). The incidence of pelvic tuberculosis is difficult to assess as many patients are asymptomatic for 10 to 20 years, therefore the disease often comes to light only incidentally during the course of investigation for a gynecological complaint (such as problems co...

Exposure to Mycobacterium tuberculosis (Mtb) aerosols is a major threat to tuberculosis (TB) researchers, even in bio-safety level-3 (BSL-3) facilities. Automation and high-throughput screens (HTS) in BSL3 facilities are essential for minimizing manual aerosol-generating interventions and facilitating TB research. In the present study, we report the development and validation of a high-throughp...

Mass spectrometry is a powerful proteomics tool for the identification of therapeutic targets and disease biomarkers on one hand characterization post-translationally modified (PTM) alternatively spliced protein isoforms other. PTMs are covalent modifications certain proteins either by addition functional groups or proteolytic cleavage. These result in structure-function regulations target gene...

As an ancient microbe, Mycobacterium tuberculosis (MTB) is an extremely successful pathogen. MTB causes more deaths worldwide now than at any previous time in history as the World Health Organization (WHO) estimates that approximately one-third of the world’s population (roughly 2 billion total) is infected with MTB [1]. MTB is a major health threat, causing 9 million new infections and between...

HIV-1 infection and HIV-1-induced immune deficiency may play a role in selecting particular Mycobacterium tuberculosis (MTB) strains (i.e. genotypes). We compared 43 MTB isolates obtained from HIV-1-infected patients with 77 MTB isolates obtained from HIV-1-uninfected patients in Burkina Faso, by means of DNA fingerprinting methods (MIRU-VNTR plus spoligotyping). This study suggests a lack of s...

BACKGROUND Human tuberculosis, which is caused by the pathogen Mycobacterium tuberculosis, remains a major public health concern. Increasing drug resistance poses a threat of disease resurgence and continues to cause considerable mortality worldwide, which necessitates the development of new drugs with improved efficacy. Thymoquinone (TQ), an essential compound of Nigella sativa, was previously...

Pseudotumour is a benign inflammatory lesion. Mycobacterial spindle cell pseudotumour (MSP) is a rare pseudotumour. It is a benign proliferation of spindle-shaped histiocytes containing acid-fast mycobacterium, commonly reported in immunocompromised patients. MSP is usually associated with mycobacterium avium complex (MAC). Here, we present the case of a 38-year-old gentleman with acquired immu...

To survive and replicate in macrophages Mycobacterium tuberculosis (Mtb) has developed strategies to subvert host defence mechanisms, including autophagy. Autophagy induction has the potential to clear Mtb, but little is known about its effect during controlled tuberculosis and HIV co-infection. Mammalian target of rapamycin complex1 (mTORC1) inhibitors were used to induce autophagy in human ma...

Pelvic tuberculosis is an infectious disease caused by the bacterium Mycobacterium tuberculosis (MTB) (95%) or Mycobacterium bovis (5%). The incidence of pelvic tuberculosis is difficult to assess as many patients are asymptomatic for 10 to 20 years, therefore the disease often comes to light only incidentally during the course of investigation for a gynecological complaint (such as problems co...

Pelvic tuberculosis is an infectious disease caused by the bacterium Mycobacterium tuberculosis (MTB) (95%) or Mycobacterium bovis (5%). The incidence of pelvic tuberculosis is difficult to assess as many patients are asymptomatic for 10 to 20 years, therefore the disease often comes to light only incidentally during the course of investigation for a gynecological complaint (such as problems co...