BACKGROUND Absorption of antigens onto chitosan microparticles via electrostatic interaction is a common and relatively mild process suitable for mucosal vaccine. In order to increase the stability of antigens and prevent an immediate desorption of antigens from chitosan carriers in gastrointestinal tract, coating onto BSA loaded chitosan microparticles with sodium alginate was performed by lay...

BACKGROUND AND PURPOSE Coil embolization is safe and effective but may be followed by aneurysm recurrence. Our purpose was to explore the use of alginate as a new embolic agent that could deliver growth factors and improve results of endovascular treatment of aneurysms. METHODS We first assessed the potential of alginate as a vector for growth factor delivery by using in vitro binding and elu...

alginate hydrogel and honey have a notably effect on wound healing process, therefore assessment of combined impact of those are essential. in this study, the effect of homey-based alginate hydrogel, alginate hydrogel and commercial alginate dressings in recovery of wound in a mouse model was studied. in this study, 20 wistar rats were divided into four groups of five. one wound with dimension ...

in recent years, encapsulation of drugs and antigens in hydrogels, specifically in calcium alginate particles, is an interesting and practical technique that was developed widespread. it is well known that alginate solution, under proper conditions, can form suitable nanoparticles as a promising carrier system, for vaccine delivery. the aim of this study was to synthesis alginate nanoparticles ...

Despite the success of alginate scaffolds and mesenchymal stem cells (MSCs) therapy in cardiac failure treatment, the impact of the physicochemical environment provided by alginate matrices on cell behavior has never been investigated. The purpose of this work was double: to determine the alginate composition influence on (1) encapsulated rat MSC viability, paracrine activity, and phenotype in...

18 Alginate lyases are enzymes that degrade alginate through β-elimination of the glycosidic bond 19 into smaller oligomers. We investigated the alginate lyases from Vibrio splendidus 12B01, a 20 marine bacterioplankton species that can grow on alginate as its sole carbon source. We 21 identified, purified, and characterized four polysaccharide lyase family 7 alginates lyases – 22 AlyA, AlyB, A...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

Alginate, a non-toxic polysaccharide isolated from brown algae, is a widely used 3-dimensional (3D) porous scaffold for the granulosa cell and follicle encapsulation. However, impurities in commercial alginate can lead to alginate biocompatibility reduction. The aim of this study was to evaluate in vitro behavior of the granulosa cells seeded on the purified alginate in varying concent...

Mucoid strains of Pseudomonas aeruginosa produce a high-molecular-weight exopolysaccharide called alginate that is modified by the addition of O-acetyl groups. To better understand the acetylation process, a gene involved in alginate acetylation called algF was identified in this study. We hypothesized that a gene involved in alginate acetylation would be located within the alginate biosyntheti...

To cause an infection, bacteriophages must penetrate the alginate exopolysaccharide of Pseudomonas aeruginosa to reach the bacterial surface. Despite a lack of intrinsic motility, phage were shown to diffuse through alginate gels at alginate concentrations up to 8% (wt/vol) and to bring about a 2-log reduction in the cell numbers in 20-day-old biofilms of P. aeruginosa. The inability of alginat...