In preparing intracellular microbial samples for one- or two-dimensional electrophoresis, trichloroacetic acid (TCA) precipitation is frequently used to remove interfering compounds. Solubilization of TCA precipitate typically requires the addition of a number of chaotropes or detergents, in a multistep process, that requires hours to carry out. In this study, a simple, rapid, one-step method t...

Alkaline treatment with steel slag and NaOH addition were investigated under different pH conditions for the fermentation of waste activated sludge. Better performance was achieved in steel slag addition scenarios for both sludge hydrolysis and acidification. More solubilization of organic matters and much production of higher VFA (volatile fatty acid) in a shorter time can be achieved at pH10 ...

Two stability indicating UV-spectrophotometric methods were developed and validated for the determination of repaglinide with its degradation products. Assay of repaglinide in 0.1M NaOH and 0.1M HCl were achieved at 216nm and 243nm respectively. The correlation was in the range of 1.0 – 25 μg mL (r = 0.9999) for 0.1M NaOH and 2.0 – 40 μg mL (r = 0.9999) for 0.1M HCl with molar absorptivity valu...

Okara is a byproduct generated during tofu or soymilk production processes. Crude polysaccharide (yield 56.8%) was isolated by removing fat, protein and low molecular weight carbohydrates from initial okara. Crude okara polysaccharide was further divided into four soluble fractions and an insoluble residue fraction by extracting with 0.05 M EDTA + NH(4) oxalate, 0.05 M NaOH, 1 M NaOH and 4 M Na...

From previous studies suggesting that controlled crystal growth and crystal dissolution are governed by the same laws, we have previously finalized the chemical lapping or controlled dissolution of AT quartz plates in NaOH,H20. The quality of chemical polishing changes with the crystallographic orientation of plates. Consequently, we have investigated simiiar basic solvents other than NaOH medi...

The study was conducted to investigate the free radical scavenging activities of polysaccharide fractions extracted from the fruiting body of medicinal mushroom, Ganoderma lucidum (Fr.) P. Karst (Reishi) using different antioxidant capacity assays. Four intracellular polysaccharide fractions were obtained by the hot-water extraction and precipitation with ethanol, by ammonium oxalate extraction...

The authors investigated a contrast enhancement behavior of hydrogen silsesquioxane HSQ in a salty development system NaOH /NaCl . Time-resolved analysis of contrast curves and line-grating patterns were carried out to investigate the unique properties of a salty development process. In NaOH developer without salt, the development process was saturated beyond a certain development time. On the ...

Coastal Bermuda grass was pretreated with NaOH at concentrations from 0.5% to 3% (w/v) for a residence time from 15 to 90min at 121 degrees C. The pretreatments were evaluated based on total lignin removal and production of total reducing sugars, glucose and xylose from enzymatic hydrolysis of the pretreated biomass. Up to 86% lignin removal was observed. The optimal NaOH pretreatment condition...

Agave americana is one of commonly grown agave species but currently less valuable because its large flower stalk cannot be used for producing alcoholic beverage. In the present study, the stalk was pretreated with dilute acid (DA), sulfite (SPORL), and sodium hydroxide (NaOH) to preliminarily assess its potential as feedstock for bioethanol production. The changes of cell wall components durin...

Low challenge phenol coefficient and high challenge use-dilution tests were made on a neutral cocoanut oil soap emulsion of o-phenylphenol and aqueous solutions of sodium o-phenylphenate prepared in the laboratory from the phenol using a stoichiometric amount of NaOH as well as with increasing amounts of excess NaOH. The phenol had considerably greater activity in both test methods when emulsif...