papillon-lefevre syndrome (pls) is a very rare genetic syndrome, and fewer than 500 cases have been reported in the world. patients exhibit typical cutaneous involvement with hyperkeratosis, especially on the soles and palms, and early shedding of primary teeth. internal organ involvements, such as liver abscesses, have been described in case reports. this  communication  represents  the  first...

we investigated the use of dna amplification by polymerase chain reaction (per) for detection of mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with pcr. two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared for dna...

Mycobacterium tuberculosis, the main cause of tuberculosis (TB), has still remained a global health crisis especially in developing countries. Tuberculosis treatment is a laborious and lengthy process with high risk of non compliance, cytotoxicity adverse events and drug resistance in patient. Recently, there has been an alarming rise of drug resistant in TB. In this regard, it is an unmet need...

We analyzed 57 publications devoted to study of genetic mechanisms formation drug resistance Mycobacterium tuberculosis bedaquiline, delamanid, pretomanid, linezolid, and clofazimine. Identification factors MTB make the basis search for new targets in development anti-tuberculosis drugs methods susceptibility testing.

Nocardiosis results from infection with bacteria of genus Nocardia, which are saprophytic aerobic actinomycetes that commonly reside in soil relatively and inactive on standard biochemical tests. Pulmonary systemic nocardiosis is common adult males, almost all being sporadic, tuberculosis endemic regions usually seen individuals deficient cell mediated immunity. It may present as pneumonia, emp...

Pulmonary nocardiosis (PN) is an infrequent and severe infection due to Nocardia spp. It is more common in immunosuppressed individual. The aim of this study was to evaluate the clinical features, evolution and prognosis of PN in immuno-competent hosts. The study group comprised 4 consecutive immuno-competent patients with pulmonary nocardiosis acquired in a community setting, diagnosed and fol...

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

methods mycobacterium tuberculosis standard strain crbip7.11 was cultured at 37°c in loewenstein johnson (lj) media for 3-4 weeks. to confirm the species, standard microbiological and biochemical tests were performed. after preparation of membrane vesicles, the amount of protein in membrane vesicles was measured by sds-page and nanodrop. to analyze the integrity and morphology of extracellular ...

background :polymerase chain reaction (pcr) assay has widely used for the detection of tuberculosis (tb). this study tried to compare in-house pcr with some well-known commercial pcr kits for detection of tb agent. methods : clinical samples obtained from 620 tb suspected patients were analyzed for the diagnosis of mycobacterium tuberculosis complex (mtc) by in-house pcr. all samples were obtai...