Nuclear reprogramming, the conversion of the epigenome of a differentiated cell to one that is similar to the undifferentiated embryonic state, can be facilitated by several methods, such as nuclear transfer, cell fusion, use of embryonic stem cell extracts, and more recently, by the introduction of exogenous transcription factors. Amongst these various strategies, somatic cell nuclear transfer...

A nuclear genome delivery system was developed to deduce the modes of inheritance of the clinical phenotypes observed in patients with mitochondrial diseases by transfer of pure nuclei from normal cells to fibroblasts from the patients. The problem of possible contamination of the nuclei with a small amount of mtDNA was overcome by using mtDNA-less (rho0) human cells as nuclear donors. In this ...

background: oral squamous cell carcinoma is the sixth most common malignancy in the world today.  ing1b/p33 is a newly-discovered tumor suppressor which enhances p53 activity. transfer of p33 protein from nucleus to cytoplasmic compartment has been previously reported in leukemias. the objective of this study was to determine the correlation between p33ing1b cytoplasmic transfer and lymph node ...

Electron spin qubits in molecular systems offer high reproducibility and the ability to self-assemble into larger architectures. However, interactions between neighboring qubits are "always on," and although the electron spin coherence times can be several hundred microseconds, these are still much shorter than typical times for nuclear spins. Here we implement an electron-nuclear hybrid scheme...

We explore theoretically the ultrafast transfer of a double electron hole between the functional groups of glycine after K-shell ionization and subsequent Auger decay. Although a large energy gap of about 15 eV initially exists between the two electronic states involved and coherent electronic dynamics play no role in the hole transfer, we find that the double hole is transferred within 3 to 4 ...

Nuclear many-body theory is based on the tenet that nuclear systems can be accurately described as collections of point-like particles. This picture, while providing a remarkably accurate explanation wealth measured properties atomic nuclei, bound to break down in high-density regime, which degrees freedom other than protons and neutrons are expected come into play. Valuable information validit...

The respiratory gene cox2, normally present in the mitochondrion, was previously shown to have been functionally transferred to the nucleus during flowering plant evolution, possibly during the diversification of legumes. To search for novel intermediate stages in the process of intracellular gene transfer and to assess the evolutionary timing and frequency of cox2 transfer, activation, and ina...

From a consideration of odd-even intramolecular electron transfer processes in octahedrally co-ordinated transition metal complexes, two relationships have been derived. The first correlation describes 71 —> y3 optical electron transfer bands of the complexes K20sC l6 , K2IrCl6 , K2PtCl6 , K2PtCl4 as a function of the respective 35C1 nuclear quadrupole coupling constants. The second rela­ tions...

Mammalian parthenogenetic embryos invariably die in mid-gestation from imprinted gene defects and placental hypoplasia. Based on chimera experiments, trophoblastic proliferation is supposed to be inhibited in the absence of a male genome. Here, we show that parthenogenetic mouse embryonic cell nuclei can be reprogrammed by serial rounds of nuclear transfer without using any genetic modification...