Nucleic acids, including DNA, microRNA (miRNA), small interfering RNA (siRNA), and antisense oligonucleotide (ASO), are powerful gene regulators, which have been demonstrated as promising drug candidates for therapeutic treatments. Nevertheless, poor cellular membrane permeability and serum stability have greatly hindered the applications of nucleic acids in biomedicine. To address these issues...

Nucleic acids promote amyloid formation in diseases including Alzheimer's and Creutzfeldt-Jakob disease. However, it remains unclear whether the close interactions between amyloid and nucleic acid allow nucleic acid secondary structure to play a role in modulating amyloid structure and function. Here we have used a simplified system of short basic peptides with alternating hydrophobic and hydro...

This minireview describes the synthesis, characterization and properties of aptamer–polymer conjugates. new class polymer bioconjugates combines advantages synthetic polymers folded nucleic acids.

BACKGROUND Microarray platforms will change immunochemical and nucleic acid-based analysis of cell homogenates and body fluids compared with classic analyses. Microarrays use labeled target and immobilized probes, rather than fixed targets and labeled probes. We describe a method for simultaneous labeling of nucleic acids and proteins. METHODS Horseradish peroxidase- and fluorescein-modified ...

Here we report efficient and selective postsynthesis labeling strategies, based on an advanced phosphoramidation reaction, for nucleic acids of either synthetic or enzyme-catalyzed origin. The reactions provided phosphorimidazolide intermediates of DNA or RNA which, whether reacted in one pot (one-step) or purified (two-step), were directly or indirectly phosphoramidated with label molecules. T...

Nucleic acids not only store genetic information in their primary sequence but also exhibit biological functions through the formation of unique structures [...]

Nucleic acids and protein prepared from Sarcoma 180, from Ehrlich ascites tumor, from NCTC 929 cells derived from strain L, and from normal mouse liver, when injected subcutaneously in mice with cultured viable NCTC 929 strain L cells, oc casionally induce cytologie atypia in the NCTC 929 cells, characterized by giant cells, abnormal cytoplasmic shapes and staining, large nuclei, and prominence...

infectious bronchitis (ib) disease is one of the important respiratory diseases of poultry that causes annually large economic losses in poultry industry of iran. the aim of this study is molecular characterization of s1 gene of iranian infectious bronchitis viruse (ibv) belonged to 793/b serotype. the whole s1 gene of three local ibv strains in rt-pcr, showed a band above 1600 base pair (bp) i...