Simultaneous separation of 37 proteinogenic D/L-amino acids excluding proline with HPLC is generally difficult. In this study, two sets o-phthalaldehyde (OPA)-based fluorescent diastereomers were formed using chiral thiols N-acetyl-L-cysteine (NAC) and N-isobutyryl-L-cysteine (NIBC) independently. It expected that obtained OPA-derivatized D/L show different retention selectivity in reversed pha...

Temporal relationship between viral and bacterial infections has been observed, and may arise via the action of virus-induced inflammatory cytokines. These, by upregulating epithelial receptors targeted by bacteria, may encourage greater bacterial infiltration. In this study, human epithelial cells exposed to interferon-gamma but not tumour necrosis factor-alpha or interleukin 1-beta supported ...

PURPOSE This study compared the measurements of intraocular pressure (IOP) and ocular pulse amplitude (OPA) using the Dynamic Contour Tonometry (DCT) over silicone hydrogel contact lenses of different modulus. Corneal biomechanics were also measured using the Ocular Response Analyzer (ORA). METHODS Forty-seven young (mean age 22.3 years, standard deviation 1.2 years) subjects had IOP, OPA, co...

PURPOSE The purpose of this study was to characterize the pressure-volume relation in the living human eye, measure the ocular pulse amplitude (OPA), and calculate the corresponding pulsatile ocular blood flow (POBF) in a range of clinically relevant IOP levels. METHODS Fifty patients with cataract (50 eyes) were enrolled in the study. After cannulation of the anterior chamber, a computer-con...

BACKGROUND/AIMS Since endoscopes are reusable apparatus classified as semicritical item, thorough reprocessing to achieve high-level disinfection is of utmost importance to prevent spread of infection. To improve disinfection efficacy and safety, disinfectants and endoscope reprocessors are continuously evolving. This study aimed to compare the efficacy of the combination of polyhexamethylenebi...

A selective and sensitive fluorometric determination method for native fluorescent peptides has been developed. This method is based on intramolecular fluorescence resonance energy transfer (FRET) detection in a liquid chromatography (LC) system following precolumn derivatization of the amino groups of tryptophan (Trp)-containing peptides. In this detection process, we monitored the FRET from t...

Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) is a highly discriminatory molecular typing procedure that provides precise and unambiguous strain characterization. Since molecular typing can complement contact tracing for reconstructing gonorrhea sexual networks, the concordance between the NG-MAST genotypes of pairs of N. gonorrhoeae isolates from recent sexual contacts was exami...

AIM This study was aimed to detect ovine pulmonary adenocarcinoma (OPA) in sheep flocks affected with pulmonary disorders at organized farm. MATERIALS AND METHODS A total of 75 sheep died naturally were thoroughly examined for the lesions of OPA during necropsy. Tissue sections from affected portion of the lungs from each animal were collected aseptically and divided into two parts; one each ...

and 50-260 SMSheppard TVInambach OELD OI-E (7) Tennessee Valley Authority BJones (8) ATTN: Mr. james E. Watson BScharf (15) Manager of Power JMcGough 818 Power Building JSaltzman Chattanooga, Tennessee 37201 CMiles, OPA TBAbernathy, DTIE Gentlemen: JRBuchanan, NSIC ACRS (16) The Commission has issued the enclosed Amendments No. 20 and 17 to Facility Licenses No. DPR-33 and i)PR-52 for the Brown...