the improvement of the physical properties of indium tin oxide (ito) layers is quite advantageous in photovoltaic applications. in this study the ito film is deposited by rf sputtering onto p-type crystalline silicon (c-si) with (100) orientation, multicrystalline silicon (mc-si), and glass substrates coated with zno and annealed in vacuum furnace at 400°c. electrical, optical, structural and m...

We describe an apertureless near field microscope for imaging fluorescent samples. Optical contrast is generated by exploiting fluorescent quenching near a metallized atomic force microscope tip. This microscope has been used to image fluorescent latex beads with subdiffraction limit resolution. The use of fluorescence allows us to prove that the contrast mechanism is indeed spectroscopic in or...

A first intermediate roll of Sendzirmir mills was failure by surface spalling during operation. After analyzing by visual, stereo microscope, optical microscope, scanning electron microscope, glow-discharged spectrometer and hardness test, respectively, the results show that some voids and cracks existed on the contact surface as well as subsurface. Further examination verified inadequate hardn...

The first electron microscope was designed in 1931 by Ernest Ruska, as his diploma thesis at the Faculty of Engineering in Berlin; it was designed on the bases of Busch’s theory about electronic lens. (Fg.1). The microscope had magnification of only 17 times. In his doctoral dissertation in 1933 Ernest Ruska developed a new electron microscope with three electromagnetic lenses. The magnificatio...

Scope of the research initiative “Jena Cell Identification Group” (JenZIG) is the combination of Raman spectroscopy with optical trapping and microfluidic chips for cell identification and sorting. Using upright microscopes, fluidic connections were realized at the bottom side [1]. Cells tend to sediment in the tubing at low flow rates which is problematic for a continuous and stable injection ...

Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. Here we describe a new SR fluorescence microscope based on confocal microscope optics, which we name the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumina...

The design, analysis, assembly methods, and optical-bench test results for a miniature injection-molded plastic objective lens used in a fiber-optic confocal reflectance microscope are presented. The five-lens plastic objective was tested as a stand-alone optical system before its integration into a confocal microscope for in vivo imaging of cells and tissue. Changing the spacing and rotation o...

Microscope has been widely used to observe microscopic objects. The types of microscope are varied according to its function. The conventional optical microscope only provides intensity information of an object and it has some difficulties to provide information about the real shape of the object and the projection of the object in different depth and perspective. In addition, optical microscop...

A high-throughput optical biosensing technique is proposed and demonstrated. This hybrid technique combines optical transmission of nanoholes with colorimetric silver staining. The size and spacing of the nanoholes are chosen so that individual nanoholes can be independently resolved in massive parallel using an ordinary transmission optical microscope, and, in place of determining a spectral s...