OBJECTIVE To compare the effects of dimethyl sulfoxide (DMSO), 1,2-propanediol (PROH), sucrose, trehalose, concentration of cryoprotectants, equilibration method, and postseeding freezing rate on doe rabbit ovarian tissue preservation after freezing, using fractional experimental design. DESIGN Experimental prospective study. SETTING Research institute in veterinary and agronomic colleges. ...

Ovarian cryopreservation and grafting has resulted in successful ovulation, pregnancy, and live-born animals including mice and sheep (1, 2). The procedure is currently being offered to humans as a new method of assisted reproduction. However, its value frequently has been questioned because of a potential to reintroduce the malignant cells, viral infections, and induction of immune responses a...

Strategies to preserve fertility in dogs and cats as cryopreservation of epididymal spermatozoa, oocytes and ovarian tissue will be discussed in this review. Recent results indicate that significant progress have been achieved, but optimal freezing protocols have not been defined yet. The extreme sensitivity of sperm membranes and of oocyte and ovarian tissue structure deserves further investig...

A surgical technique has been established for orthotopic transplantation of ovarian tissue in newly hatched chickens. In these trials survivability of the chicks after surgical manipulation was 100%. The size and orientation of the ovary at 2 wk of age suggested that the graft was attached and had undergone development. This technique should allow the development of a cryopreservation protocol ...

abstract ovarian tissue freezing or cryopreservation might be the only acceptable method for preserving the young women fertility, before radiotherapy or chemotherapy. this technology might be used for patients with recurrent ovarian cysts or endometriosis, without ovarian stimulation. many efforts have made to improve cryopreservation conditions that should be seriously considered for cancer p...

BACKGROUND Cryopreservation of ovarian tissue has been widely accepted as an option for fertility preservation among cancer patients. Some patients are exposed to chemotherapy prior to ovarian tissue cryopreservation. Consequently, assessment of the developmental capacity of human ovarian tissue after chemotherapy is of primary importance. MATERIALS In order to study the impact of previous ch...

background: many attempts have done to improve cryopreservation of mammalian ovaries using simple, economical and efficient technique “vitrification”. objective: the aim of the present study was to compare the mouse ovaries cryopreservation by direct cover vitrification (dcv) using different concentrations of ethylene glycol (eg) with conventional vitrification methods (cv). materials and metho...

BACKGROUND Ovarian tissue cryopreservation is the only option for preserving fertility in prepubertal girls and cancer patients requiring immediate treatment. Following ovarian tissue cryopreservation, fertility can be restored after tissue transplant or in vitro follicle maturation. METHODS Macaque (n= 4) ovarian cortex was cryopreserved using slow-rate freezing (slow freezing) or vitrificat...