Many nonhematologic tumors produce growth factors that may influence cellular proliferation either by autocrine or by paracrine mechanisms. In the current study, human tumor cell lines were investigated for the constitutive production of macrophage colony-stimulating factor (M-CSF). Culture supernatants obtained from cell lines were analyzed using a radioimmunoassay and a radioreceptor assay sp...

PURPOSE The presence of similar histologic subtypes of epithelial ovarian and endometrial cancers has long been noted, although the relevance of this finding to pathogenesis and clinical management is unclear. Despite similar clinical characteristics, histologic subtypes of cancers of the ovary and endometrium are treated according to organ of origin. This study compares the gene expression pro...

This is a survey of the work intended to contribute to our knowledge of normal gonadal sex differentiation in birds and mammals. Special attention has been given to the fate of germ cells in a foreign environment. The original work was undertaken to support the concept of a meiosis-inducing substance. Gonads were combined in heterosexual pairs and grown in organ culture. Homoplastic, heteroplas...

Organ cultures of human embryonic trachea in test tubes were used as an adjunct to tissue cultures in the isolation of respiratory viruses from children in hospital. Fifty-one viruses were obtained from 127 specimens, giving an isolation rate of 40%. Fifteen viruses were isolated from the original tissue cultures and also after passage through organ culture. Thirty viruses were isolated from th...

Background: Currently clomiphene citrate is the first-line treatment to induce ovulation in women with polycystic ovarian syndrome (PCOS). Surgical therapy with laparoscopic ovarian drilling (LOD) may avoid or reduce the need for gonadotropins. Objective: To determine the effectiveness and safety of LOD compared with ovulation induction in subfertile women with clomiphene-resistant PCOS. Search...

The synthesis of yolk proteins can conveniently be studied in Octopus where removal of the subpedunculate lobe from the brain causes secretion of a gonadotropin by the optic glands and results in precocious sexual maturation (Wells & Wells, 1959). Maturing animals can thus be prepared as needed. The normal reproductive pattern of the female is a further advantage. All the ova from any single an...

Effect of co-culture of buffalo preantral follicles with different somatic cells, i.e. cumulus cells, granulosa cells, Ovarian Mesenchymal Cells (OMC) and Oviductal Epithelial Cells (OEC) was studied. Large preantral follicles were isolated by microdissecting the trypsin (1%) digested ovarian cortical slices. Cumulus cells were isolated by repeated pipetting of oocytes, granulosa cells were iso...

Mesenchymal stem cells (MSCs) secrete a variety of cytokines and growth factors in addition to self-renewal and multiple forms of differentiation. Some of these secreted bioactive factors could improve meiotic maturation in vitro and subsequent embryo developmental potential. The aim of the present study was to determine whether in vitro maturation (IVM) of mouse oocyte with or without cumulus ...

Testing cardiac gene and cell therapies in vitro requires a tissue substrate that survives for several days in culture while maintaining its physiological properties. The purpose of this study was to test whether culture of intact cardiac tissue of neonatal rat ventricles (organ explant culture) may be used as a model to study gene and cell therapy. We compared (immuno) histology and electrophy...