The b2 structures of model systems Xxx-Flp-Ala (Flp = 4R-fluoroproline) and Xxx-flp-Ala (flp = 4S-fluoroproline) (where Xxx is Val or Tyr) were studied by action IRMPD spectroscopy. Proline ring substitutions influence the trans/cis isomerization of the precursor ion, resulting in different b2 fragment ion structures by collision induced dissociation. Vibrational spectra of the b2 ions of Val-F...

Mucosal eosinophils increase in a number of gastrointestinal diseases that are often associated with altered epithelial barrier function, including food allergic enteropathies and inflammatory bowel diseases. Although eosinophils are known to secrete biologically active mediators including granule proteins, their role in gastrointestinal diseases is uncertain. The aim of this study was to deter...

background and objectives: baneh tree or pistacia atlantica subsp. kurdica is an endemic plant of iran which belongs to anacardiaceae family. it has various traditional uses including astringent and anti-diarrheal as well as improving some of the symptoms of gastrointestinal upsets. in this study we decided to investigate the effects of various fractions of baneh gum with different doses in an ...

Charge-directed fragmentation has been shown to be the prevalent dissociation step for protonated peptides under the low-energy activation (eV) regime. Thus, the determination of the ion structure and, in particular, the characterization of the protonation site(s) of peptides and their fragments is a key approach to substantiate and refine peptide fragmentation mechanisms. Here we report on the...

Animal models mimicking human diseases have been used extensively to study the pathogenesis of autoimmune diseases and the efficacy of potential therapeutics. They are, however, limited with regard to their similarity to the human disease and cannot be used if the antagonist and its cognate receptor require high similarity in structure or binding. Here, we examine the induction of oxazolone-med...

Ulcerative colitis (UC) is a chronic and non-specific inflammatory bowel disease (IBD). Its pathogenesis remains unclear, but its morbidity shows an increasing trend year by year. The of UC may be related to the genetic susceptibility, immune factor intestinal microflora. In last few years, number studies have examined relationship between expression levels peripheral CircRNA UC. We aimed evalu...

By screening 720 natural compounds in a standard 2-way allogeneic mixed leukocyte reaction assay, we identified a potent immunosuppressive capacity of crassin acetate (CRA), a coral-derived cembrane diterpenoid. CRA efficiently inhibited allogeneic mixed leukocyte reaction as well as antigen-specific activation of CD4 T cells by bone marrow-derived dendritic cells (DCs). With regard to cellular...

The pathogenesis of inflammatory bowel diseases has not been fully studied, and the therapies used have side effects that limit their use. purpose this study is to conduct a clinical immunological analysis effectiveness vitamin D 3 in original rectal suppositories experimental colitis (EC). EC was modeled with oxazolone. Original group 5-ASA 4 were per rectum . clinic evaluated on Disease activ...

Objective(s):Inflammatory bowel disease (IBD) results from dysregulation of intestinal mucosal immunity. It is an incurable disease that affects millions of people worldwide. Developing new strategies for the treatment of colitis has been a major challenge. Here, we report the effect of calycosin, a plant-derived flavonoid, in successfully managing colitis in murine model. Material and Methods:...

aim : to induce acute colitis progresses to chronicity in c57bl/6 mice by dextran sulfate sodium.   background : murine models are essential tools to understand ibd pathogenesis. among different types of chemically induced colitis models, the dextran sulfate sodium (dss)-induced colitis model is the most common model of ibd, due to its simplicity. patients and methods : male c57bl/6 mice 6–8 we...