Lytic polysaccharide monooxygenases (LPMOs) are a class of powerful oxidative enzymes that breakdown recalcitrant polysaccharides such as cellulose. Here we investigate the action of LPMOs on cellulose fibers. After enzymatic treatment and dispersion, LPMO-treated fibers show intense fibrillation. Cellulose structure modifications visualized at different scales indicate that LPMO creates nickin...

Marine natural products are a source of promising agents for cancer treatment. However, there is a need to improve the evaluation of their mechanism of action in tumors. Metabolomics of the response to anti-tumor agents is a tool to reveal candidate biomarkers and metabolic targets. We used two-dimensional high-resolution magic angle spinning proton-NMR spectroscopy-based metabolomics to invest...

Tyrosinase, which usually catalyzes the conversion of o-diphenols to o-benzoquinones, catalyzed an unusual oxidative dimerization of 1,2-dehydro-N-acetyldopamine to a benzodioxan derivative. The identity of the product was confirmed by UV, IR spectra, and NMR studies. During the oxidation, generation of a transient reactive intermediate could be witnessed by its characteristic visible absorptio...

Parkinson's disease (PD) is a neurodegenerative disease, which is characterized by progressive death of dopaminergic neurons in the substantia nigra pars compacta. Although mitochondrial dysfunction and oxidative stress are linked to PD pathogenesis, its etiology and pathology remain to be elucidated. Metabolomics investigates metabolite changes in biofluids, cell lysates, tissues and tumors in...

0146-6380/$ see front matter 2008 Elsevier Ltd doi:10.1016/j.orggeochem.2008.06.006 * Corresponding author. Tel.: +1 423 439 6917; fa E-mail address: [email protected] (Y.L. Jiang). A new and convenient fluorescence spectroscopic method has been developed for the separation and determination of perylene in fossil wood recovered from a late Miocene to early Pliocene Gray Fossil Site in Tennessee, ...

TFF3 regulates essential gastro- and neuroprotective functions, but its molecular mode of action remains poorly understood. Synthetic intractability lack reliable bioassays validated receptors are bottlenecks for mechanistic structure–activity relationship studies. Here, we report the chemical synthesis homodimer via native ligation followed by oxidative folding. Correct folding was confirmed N...

The control of oxidative metabolism was studied using carbon-13 (13C) nuclear magnetic resonance (NMR) spectroscopy to determine the ratelimiting steps in 13C labeling of glutamate. 13 C NMR spectra were acquired from isolated rabbit hearts perfused with either 2.5 mM [213C] acetate or 2.5 mM [213 C] butyrate, and with or without KCl arrest. The tricarboxylic acid (TCA) cycle flux and the inter...

Objective: Alchornea floribunda leaves are widely used in ethnomedicine for the management of immuno-inflammatory disorders. We investigated the in vivo and in vitro antioxidant activity of the leaf extract, fractions and isolated compounds of A. floribunda.Materials and Methods: The ethyl acetate fraction of the methanol leaf extract was subjected to several chromatographic separations to isol...

The hamster has been previously found to be a suitable model to study the changes associated with diet-induced hyperlipidemia in humans. Traditionally, studies of hyperlipidemia utilize serum- or plasma-based biochemical assays and histopathological evaluation. However, unbiased metabonomic technologies have the potential to identify novel biomarkers of disease. Thus, to obtain a better underst...

Two new copper(II) complexes of bidentate arylhydrazone ligands have prepared and studied. Elemental analysis, SEM-EDS, thermal gravimetric analysis (TGA), powder X-ray diffraction (XRD), a number spectroscopic techniques were used to determine the structures compounds (FTIR, UV-Vis, 1H 13C-NMR). Using agarose gel electrophoresis, DNA cleavage activities produced copper investigated in absence ...