Here, we present the solution structure of a DNA duplex containing a disulfide base pair (S-DNA). The unnatural nucleoside "S" possessing a thiophenyl group as base was incorporated into a self-complementary singled-stranded oligonucleotide. Crosslinking of the disulfide base pair was analyzed by non-denaturing polyacrylamide gel electrophoresis. Under oxidizing conditions a high molecular weig...

Chick embryo corneas (stages 38 and 45) have been used to study variations in pentose shunt activity following the use of a glutathione-specific oxidizing agent, diamide, and a sulfydryl blocking agent, N-ethylmaleimide (NEM). Shunt activity was measured by the ratio of radiolabeled carbon 1 (14C-1) of glucose to radiolabeled carbon 6 (14C-6) of glucose derived as expired 14CO2. Diamide and NEM...

The residues of persistence potentially toxic organic compounds polyaromatic hydrocarbons (PAHs) were measured in 13 species of edible vegetables collected from Great Cairo governorate (Egypt). And the efficient role of washing by oxidizing agents hydrogen peroxide (H O ), potassium permanganates (KMnO ) 2 2 4 and midribs of cabbage leaves solutions on the elimination of PAHs from contaminated ...

The publications on Kjeldahl’s method for determining nitrogen that have appeared since Kjeldahl’s (1) original paper in 1883 perhaps outnumber those on any other analytical method in the same period of time.1 The diversity of papers is attributable to the immense usefulness of the method, to its need for modifications for applications to various types of organic and inorganic compounds, and to...

Granulocytes engaged in the phagocytosis of opsonized zymosan emit light by a process that is inhibited by superoxide dismutase and catalase. In the present report is is shown that light emission is the result of reactions between certain unspecified constituents of the ingested particles and some or all of the oxidizing agents (H2O2, O2),and possibly the hydroxyl radical and singlet oxygen) pr...

A spectrophotometric method is proposed to determine isoniazid using oxidative coupling reaction. The depends on adding the oxidizing agent potassium periodate reagent solution 2,4-dinitrophenylhydrazine. Different optimum conditions are studied and explained based various experiments tests. wavelength (406) nm. Beer's law within concentration (0.2-40) µg/ml, with a molar absorptivity value of ...

Microwave-activated synthesis is a special and remarkable technique in modern synthetic organic chemistry. In the current research, it described microwave-assisted of 2-aryl-4,5-dicarbonitrile imidazole derivatives via one-step reaction between aromatic aldehydes 2,3-diaminomaleonitrile (DAMN) using HNO3 as metal-free catalyst strong oxidizing agent. The present methodology has significant adva...