Vicinal aminoalcohols are widespread structural motifs in bioactive molecules. We report the development of a new dioxazolone reagent containing p-nitrophenyldifluoromethyl group, which 1. displays good safety profile; 2. shows remarkably high reactivity oxime-directed iridium(III)-catalyzed amidation unactivated C(sp3)−H bonds; 3. leads to amide products can be hydrolyzed under mild conditions...

Organophosphorus compounds (OPs) are potent inhibitors of acetylcholinesterase (AChE). Treatment for OP poisoning is by administration of atropine sulfate, an oxime, and diazepam. Oximes such as 2-PAM are used to reactivate OP-inhibited AChE so as to restore normal enzymatic function and serve as a true antidote. There are reports of non-enzymatic hydrolysis by oximes of acetylthiocholine in in...

Cassava (Manihot esculenta) is a eudicotyledonous plant that produces the valine- and isoleucine-derived cyanogenic glucosides linamarin and lotaustralin with the corresponding oximes and cyanohydrins as key intermediates. CYP79 enzymes catalyzing amino acid-to-oxime conversion in cyanogenic glucoside biosynthesis are known from several plants including cassava. The enzyme system converting oxi...

In the present paper we show a comprehensive in vitro, ex vivo and in vivo study on hydrolytic detoxification of nerve agent and pesticide OPs (organophosphates) catalysed by purified hBChE (human butyrylcholinesterase) in combination with novel non-pyridinium oxime reactivators. We identified TAB2OH (2-trimethylammonio-6-hydroxybenzaldehyde oxime) as an efficient reactivator of OP-hBChE conjug...

Silicon and germanium containing pyridine aldoxime, ketoxime and amidoxime O-ethers have been prepared using phase transfer catalytic systems oxime alkyl halide solid KOH 18-crown-6 benzene and oxime alkyl halide solid K(2)CO(3) or Cs(2)CO(3) 18-crown-6 toluene. Cytotoxic activity of silicon and germanium containing pyridine oxime O-ethers was tested in vitro on two monolayer tumor cell lines: ...

Heavy metal contamination still prevails due to improper discharge of effluents from industries, mining activities, agricultural and domestic sources. Hence, the objectives this study were isolate bacteria fungi with high potentials for biosorption Cd(II), Pb(II), Cr(III) Ni(II) explore their functional groups. Bacteria able grow in presence 0.1% selected heavy metals isolated enumerated using ...

testosterone was measured using antibodies raised against testosterone ii b-carboxymethyl ether bovine serum albmin (t-iib-cme-bsa) and testosterone 3- o-carboxymethyl oxime-bsa as immunogen. the antibody produced in this study exhibits minimal cross reactivity with the structurally related steroids specially 5 dihydrotestosterone (5 dhd. this allows to ommit the clean up step and measure testo...

The biogenic amine phenethylamine has been shown to be N-oxygenated by human flavin-containing monooxygenase (FMO) (form 3) and human liver microsomes and, to a much lesser extent, N-oxygenated by porcine liver FMO1 and porcine liver microsomes but not by rabbit FMO2. Adult human liver microsomes catalyze the NADPH-dependent N-oxygenation of phenethylamine to the corresponding trans-oxime throu...

The synthesis of visnaginone-oxime (2 a) and khellinone-oxime (2 b) is reported. Beckmann rearrangement of 2 a and 2b using different cyclising agents was studied. Structure assignement for the produced benzoxazole derivatives and benzisoxazole derivatives was confirmed by chemical and spectral evidences. Utilization of visnaginone (la) and khellinone (lb) for the synthesis of new furoflavone d...