A leptospirose é uma zoonose de grande importância em saúde pública. Cento e quatro (104) amostras urina bovinos abatidos na região centro-oeste do Estado São Paulo, Brasil, foram avaliadas para detectar Leptospira spp. As mantidas PBS culturas EMJH Fletcher. Observamos crescimento microrganismos 13 meio Fletcher, embora não tenha sido observado no EMJH. Por PCR, 25 animais (amostras) positivos...

Man is so much in contact with animals that it is not surprising that at times he contracts disease from them. Much work remains to be done on diseases in which the part an animal plays is still unknown. It is impossible to cover the ground of all diseases which either directly or indirectly are associated with animal contacts, but the following Table (I) gives an indication of some of them. Ma...

background: leptospirosis has been recognized as an important reemerging infectious disease caused by pathogenic leptospira spp. a major challenge of this disease is the application of a basic research to improve diagnostic method. outer membrane proteins of leptospira are potential candidates that could be useful in diagnosis. among them the lipl41 is an immunogenic protein which is present on...

A protein-free chemically defined medium for cultivation of pathogenic Leptospira was developed. The medium permitted continued serial subculturing of 9 serogroups (52 strains) of the 12 serogroups (61 strains) tested. Growth was initiated from small inocula, and the growth rate and maximal cell yields were similar to those on serum-containing media. The nutritional requirements of serogroups L...

The gene encoding the 5S rRNA for Leptospira interrogans serovar canicola strain Moulton was isolated and sequenced. The 5S rRNA gene occurs as a single copy within the genome and encodes a 117-nucleotide-long RNA molecule. The 5S rRNA gene is flanked at both the 5' and 3' ends by regions of A + T-rich sequences, and the 5'-flanking region contains a promoter sequence. L. interrogans has a uniq...

This study aimed to assess the exposure of free-living jaguars (Panthera onca) to Leptospira spp. and Brucella abortus in two conservation units in the Pantanal of Mato Grosso, Brazil. The presence of antibodies in blood samples of eleven jaguars was investigated using autochthonous antigens isolated in Brazil added to reference antigen collection applied to diagnosis of leptospirosis by Micros...

INTRODUCTION The incidence of canine leptospirosis in Brazil needs to be assessed. METHODS The same dogs in southern Brazil were sampled over two years to determine the prevalence, incidence and association of canine leptospirosis with various risk factors. RESULTS In 2009, the prevalence was 33 (14.4%) of 228 dogs, with a predominance of serovar Canicola (33.4%). In 2010, 90 dogs were re-e...

The epidemiologic status of leptospirosis in Egypt has not been well defined because of difficulties in disease diagnosis. A retrospective study was conducted to detect leptospiral antibodies among undiagnosed acute febrile illness (AFI) and hepatitis cases. Approximately 16% of both AFI (141/886) and acute hepatitis (63/392) cases showed seroreactivity to Leptospira IgM by ELISA and microscopi...