To reduce the window period between HIV-1 infection and the ability to diagnose it, a fourth-generation immunoassay including the detection of HIV-1 p24 antigen has been developed. However, because the commercially available systems for this assay use special, high-cost instruments to measure, for example, chemiluminescence, it is performed only by diagnostics companies and hub hospitals. To ov...

Aim: The present study aimed to detect Human bocavirus (HBoV) in stool samples from young children below 5 years complaining of acute gastroenteritis (GE) addition detection rotavirus, norovirus, and astrovirus. Methods: included 90 with GE excluded bacterial pathogens. determination the presence HBoV was performed by nested polymerase chain reaction (PCR) beside astrovirus norovirus multiplex ...

INTRODUCTION Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and enzyme-linked immunosorbent assay (ELISA) are commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study w...

Neonatal diarrhea induced by bovine group A rotavirus causes significant economic loss in the dairy and beef industry due to increased morbidity and mortality, treatment costs, and reduced growth rates. The objective of this study was to evaluate a human group A rotavirus assay (ImmunoCardSTAT Rotavirus [ICS-RV]) as an on-site diagnostic test for bovine rotavirus. When used with a collection of...

In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For purpose, 100 dog uteruses of ages breeds, were removed by ovariohysterectomy their blood samples used as study material. Enzyme-linked immunosorbent assay (ELISA) Indirect Fluorescent Antibody Test (IFAT) for serological diagnosi...

patients and methods five hundred and sixteen serum samples of the patients undergoing hemodialysis and kidney transplantation from various cities of khuzestan province were collected. anti-hepatitis g e2 antibodies were investigated by elisa method. rnas were extracted from serums and hepatitis g rna was detected by rt-pcr. results of the 516 samples, 38 (7.36%) specimens were positive for ant...

Background Chlamydia trachomatis (CT) with damaging effects on sperm quality parameters can often cause infertility in men. Objective The main objective of this study was to determine the diagnostic value of polymerase chain reaction (PCR) and enzyme linked immuno sorbent assay (ELISA) for screening and detecting CT in semen samples of infertile men. Materials and Methods In this cross-sect...

In this study, detection of BHV-1 infections in cattle by using polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) investigation the risk factors associated with spread virus within herd were aimed. Three dairy farms selected Thrace district, Marmara region, Turkey, two visits to these farms. A questionnaire was prepared for farmers. On first visit, 4 (14.81%) 27 anim...

Introduction. The ability of SARS-CoV-2 antibodies to neutralize the virus is primary indicator their specific activity. test for neutralizing (NAbs) much needed in different biomedical studies.The aim study find optimum conditions microscopic and spectrophotometric detection SARSCoV-2 NAbs by inhibition cytopathic effect (CPE) cell cultures.Materials methods. Blood sera collected from COVID-19...

an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...