Background: Formation of secondary structure such as DNA hairpins or loops may influence molecular genetics methods and PCR based approaches necessary for genetic engineering, in addition to gene regulation. Materials and Methods: A polymerase chain reaction with splice overlap extension (SOE-PCR) was used to create fully synthetic 1F5 chimeric anti-CD20 heavy- and light-chain genes. The chi...

Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...

this study was conducted to detect the common bacteria implicated in bovine mastitis in bulk tank milk by polymerase chain reaction (pcr). forty-four milk samples from bulk tank milk were obtained and submitted to our laboratory. to detect staphylococcus aureus, streptococcus agalactiae, streptococcus dysgalactiae, escherichia coli, streptococcus uberis, and streptococcus parauberis, two sets o...

Pada masa pandemi Covid-19, teknik penggandaan DNA dengan metode Polymerase Chain Reaction (PCR) menjadi gold standard untuk diagnosis virus dan penghitungan sampel klinis. Metode PCR ini diimplementasikan pada alat nama Thermal Cycler atau mesin PCR. Salah satu komponen utama dalam adalah pemanas. Tiga buah pemanas, yakni: Peltier Device 12715, Ceramic Heater PTC 12 VDC, Customized VDC diuji k...

results 162 yeast species from 250 specimens were isolated in sabroud dextrose agar (sda) medium. 106 (65.4%) of them were germ tube formation positive, 86 (53%) chlamydoconidium formation positive and 101(62.4%) were pcr positive. yeast cells and mycelia were detected in the isolates on direct microscopic examination. c. albicans accounted for 66.04% of cases and 34% were non-c. albicans speci...

Molecular techniques based on the polymerase chain reaction (PCR) can provide rapid and sensitive diagnosis of plum pox virus (PPV), the causal agent of the devastating 'sharka' disease of stone fruit trees. The present study compared routine polymerase chain reaction (PCR) procedures against a new system, PCR-ELISA (Boehringer Mannheim), which enables immunoenzymatic detection of PCR products....

AIM To develop a method for enhanced polymerase chain reaction (PCR) product detection. METHODS During the PCR, the double-stranded product is generated with fluorescent dye on one strand, and biotin on the other strand. The product is captured on the streptavidin-coated plates with high efficiency (IPCRp). Washing of the all unamplified compounds, including dye-labeled unincorporated primers...

background neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years, and it is characterized by wide clinical and biological heterogeneity. n-myc oncogene amplification is considered to be one of the most important prognostic factors used to evaluate survival in these patients. objectives the aim of our study was to determine amplification of the n-myc oncog...

Background & Aims: Calcifying nanoparticles are different forms of calcium and phosphate in sediments. Recent evidence suggests that calcifying nanoparticles (CNPs) are probably selfreplicating. Several diseases are linked to nano-bacteria including kidney stones, gallbladder stone, cardiovascular plaques, oral–dental plaque, many cancers, and autoimmune diseases. The aim of this study was to a...