Continuous production of nisin in laboratory media and whey permeate was investigated using a packed-bed bioreactor. Lactococcus lactis subsp. lactis ATCC 11454 was immobilized by natural attachment to fibre surfaces and entrapment in the void volume within spiral wound fibrous matrix. The inoculated bioreactor was fed M17 medium and effect of pH, temperature, dilution rate, and substrate conce...

BACKGROUND: Use of lactose-rich concentrates from dairy processes for the induction of recombinant gene's expression has not received much attention although they are interesting low cost substrates for production of recombinant enzymes. Applicability of dairy waste for induction of recombinant genes in Escherichia coli was studied. Clones expressing Lactobacillus phage muramidase and Lactobaci...

Numerous researches have been investigated on the mass transfer phenomena and hydrodynamics for the fluid in the vicinity of the membrane surface by the mathematical modelling and simulation. Due to complexities involved in solving transport phenomena within membranes, the application of CFD simulation study for determining the concentration polarization (CP) profile in the membrane channel is ...

A pilot plant using ceramic microfiltration membranes which could be regularly back-flushed with air for fouling control was evaluated for the treatment of spent cutting-oil. It was found that membrane fouling could be effectively controlled with regular air back-flushes (every 3 min for 1 s) and chemical cleaning using caustic soda. Permeate flux remained approximately constant and varied from...

Direct Capillary Nanofiltration (Direct CNF) is a new technique used for surface water and wastewater treatment in one step without pre-treatment. The CNF membrane module combines the favourable cleaning properties of capillary ultrafiltration membranes with the favourable separation properties of nanofiltration membranes in terms of removal of DOC, colour, bacteria, viruses and pesticides. Dir...

This work deals with the development of a new bioprocess for the efficient synthesis of lactosucrose, a potential prebiotic oligosaccharide with a high value-added, from two important and inexpensive agro-industrial by-products such as tofu whey and cheese whey permeate. The bioconversion is driven by the ability of the enzyme levansucrase SacB from Bacillus subtilis CECT 39 to transfructosylat...