Peroxidase enzyme plays an important role in pharmaceutical, Industrial and various research laboratories. This enzyme can be purified by various different method. Our research trend was to purify the enzyme in a convenient and easy way. For this purpose a new affinity column was prepare by immobilizing PABA to N-HydroxySuccinamaideagarose gel. The presence of peroxidase was investigated on var...

2-Cys Prxs are H2O2-specific antioxidants that become inactivated by enzyme hyperoxidation at elevated H2O2 levels. Although hyperoxidation restricts the antioxidant physiological role of these enzymes, it also allows the enzyme to become an efficient chaperone holdase. The critical molecular event allowing the peroxidase to chaperone switch is thought to be the enzyme assembly into high molecu...

A diverse collection of actinomycete strains were screened for production of extracellular peroxidase activity by adapting a chemiluminescence analysis system developed for horseradish peroxidase-based enzyme-linked immunosorbent assay. Extracellular peroxidase activity was found to be common but quantitatively variable, and this rapid and sensitive screening system permitted identification of ...

Nowadays the treatment of environmental pollutants such as synthetic dyes (used in multiple industries paper, textile, food, plastic and pharmaceutical) has received much attention, especially for biotechnological treatments using both native artificial enzymes. In this context, many enzymes have been reported to efficiently perform dye degradation. Peroxidase is one enzyme, which causes degrad...

The lutropin-induced depletion of ascorbate in corpora lutea of albino-rat ovary is shown to be associated with the induction of peroxidase in corpora lutea. An inverse relationship between ascorbate depletion and peroxidase activity was established in a time-course study with lutropin. Analyses made at different phases of the reproductive cycle are in accord with this relationship. It is sugge...

Rotavirus infections in intestinal tissues of animals or in tissue culture cells were detected by the immunocytochemical unlabeled soluble enzyme peroxidase antiperoxidase method. Comparison of the immunofluorescence and peroxidase antiperoxidase immunological staining techniques revealed that the two methods are equally sensitive for detection of rotavirus-infected cells. The peroxidase antipe...