1050 BioTechniques Vol. 26, No. 6 (1999) DNA in solution were used instead of 10.75 μL of ddH2O and 8.5 μL of DNA in solution. The re-amplified eluted band was electrophoresed, gel-purified and cloned into a pGEM-T vector (Promega, Madison, WI, USA). We then identified clones containing recombinant plasmids, isolated plasmid DNA and used the insert of an individual clone as a hybridization pro...

BACKGROUND & OBJECTIVES The unique immunological functions of γδ T lymphocytes to contribute immunity against Mycobacterium tuberculosis attracted interest of researchers. However, little is known about the specificity of γδ Τ cell in tuberculosis patients and the lack of exact tuberculosis antigen recognized by γδ T cells limited its application. The analysis of complementary determinant regio...

Studies using culture dependent methods have indicated that enterococci, the fecal indicator used to monitor marine waters for the potential of enteric disease risk to swimmers, can be abundant in beach sands and may contribute to water column indicator exceedances. A quantitative PCR (qPCR) method for the Enterococcus genus was tested and applied to more rapidly determine the amount of enteroc...

Chrysanthemum stunt viroid (CSVd), a noncoding infectious RNA molecule, causes seriously economic losses of chrysanthemum for 3 or 4 years after its first infection. Monomeric cDNA clones of CSVd isolate SK1 (CSVd-SK1) were constructed in the plasmids pGEM-T easy vector and pUC19 vector. Linear positive-sense transcripts synthesized in vitro from the full-length monomeric cDNA clones of CSVd-SK...

Burn injuries result in the release of proinflammatory mediators causing both local and systemic inflammation. Multiple organ dysfunctions secondary to systemic inflammation after severe burn contribute to adverse outcome, with the lungs being the first organ to fail. In this study, we evaluate the anti-inflammatory effects of Parecoxib, a parenteral COX-2 inhibitor, in a delayed fluid resuscit...

apple scar skin viroid (assvd) is one of the most destructive viroid diseases in pome fruits orchards which can cause significant economic losses. symptoms of this viroid including scaring, distortion and dappling were seen in some apple orchards in east-northern of iran. sampling was carried out from apple and pear orchards. the rna was extracted by silica capture method from leaves. assvd was...

Background Eucalyptus genetic improvement is time-consuming mainly because its long time to reach reproductive maturity along with its mixed mating system [6]. As a result, the production of pure lines as in maize is impracticable. The heterozygous status hides deleterious recessive genes that, when in homozygosity can reduce seedling survival due to physiological changes, resulting in consider...