We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation. The pHlash sensor-composed of a donor luciferase that is genetically fused to a Venus fluorophore-exhibits pH dependence of its spectral emission in vitro. When expressed in either yeast or mammalian cells...

The main aim of this paper is to propose a state of art wireless sensor technology in agriculture, which can show the path to the rural farming community to replace some of the traditional techniques. In this project, the sensor motes have several external sensors namely leaf wetness, soil moisture, soil pH, atmospheric pressure sensors attached to it. Based on the value of soil moisture sensor...

Foot ulcers account for 15% of comorbidities associated with diabetes. Presently, no device allows the status of foot ulcers to be continuously monitored when patients are not hospitalized. In this study, we describe a temperature and a pH sensor capable of monitoring diabetic foot and venous leg ulcers developed in the frame of the seventh framework program European Union project SWAN-iCare (s...

A highly selective electrode for Bromide ion based on aza pyrilium derivative as an excellentionophore is described.The sensor exhibits a good linear response with a slope of ( 60±1 ) mV per decadeover the concentration range of ( 1×10-3 – 9×10-6 M ) , and a detection limit of ( 3×10-6 M ) of Bromideions .The electrode response is independent of pH in the range of(4.0 –9.5).Selectivity coeffici...

A new class of rhodamines for the application as indicator dyes in fluorescent pH sensors is presented. Their pH-sensitivity derives from photoinduced electron transfer between non-protonated amino groups and the excited chromophore which results in effective fluorescence quenching at increasing pH. The new indicator class carries a pentafluorophenyl group at the 9-position of the xanthene core...

The pH sensing and nonideal characteristics of a ruthenium nitride (RuN) sensing membrane pH sensor were investigated. RuN thin films were deposited from a 99.9% ruthenium target on p-type silicon substrates using radio frequency (r.f.) sputtering with N(2) gas. Subsequently, the nanometric structure and surface morphology of RuN thin films were determined. The sensitivity of the RuN sensing me...

The influence of the Ar/O2 gas ratio during radio frequency (RF) sputtering of the RuO2 sensing electrode on the pH sensing performance is investigated. The developed pH sensor consists in an RF sputtered ruthenium oxide thin-film sensing electrode, in conjunction with an electroplated Ag/AgCl reference electrode. The performance and characterization of the developed pH sensors in terms of sens...

The ̄uorescent and halide sensitive 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) molecule has been synthesised and incorporated into a hydrophilic copolymer to produce a halide sensor ®lm. The sensor ®lm typically swells in aqueous media allowing dye ̄uorescence to be quenched by the diffusion of aqueous halide ions at high pH. The steady-state Stern±Volmer constants, KSV, for the sensor ®lm to...

HIV-1 fusion leading to productive entry has long been thought to occur at the plasma membrane. However, our previous single virus imaging data imply that, after Env engagement of CD4 and coreceptors at the cell surface, the virus enters into and fuses with intracellular compartments. We were unable to reliably detect viral fusion at the plasma membrane. Here, we implement a novel virus labelin...

A feasible and fast method to fabricate hydrogen peroxide sensor was investigated by graphene-manganase nanocomposite carbone paste electrode. In the present work, in first step, the graphene was synthesized by chemical method and in second step, manganese oxide nanoparticle was doped on graphene. graphene-manganase nanocomposite was characterized by FTIR and SEM. The nanocomposite shows a high...