Water is ubiquitous in the mouths of healthy individuals and is a major interfering factor in the development of a durable seal between the tooth and composite restoration. Water leads to the formation of a variety of defects in dentin adhesives; these defects undermine the tooth-composite bond. Our group recently analyzed phase partitioning of dentin adhesives using high-performance liquid chr...

Super/subcritical Fluid Chromatography (SFC) has been used successfully for the analysis of a variety of compounds including foods, polymers, and pharmaceuticals. 1-7 Supercritical carbon dioxide exhibits solvating power comparable to hexane and has been primarily utilized as a fluid for the separation of relatively non-polar compounds. 8 For this reason, many SFC methods have been developed as...

The triacylglyceride components of cottonseed oil were isolated and positively identified by a combination of high performance liquid chromatography (HPLC) and gas chromatography (GC). Both reversed-phase HPLC and capillary GC were capable of separating the oil into triacylglyceride peaks. These peaks were isolated by HPLC and their component acyl groups were converted to fatty acid methyl este...

Rheum species are medicinally important herbs, posses several type of biological activities due to the presence of anthraquinone derivatives. Several HPLC methods have been used for sepeatation of anthraquinone of Rheum Species. In most cases anthraquinones were separated using C18 column as a stationary phase, acetonitrile, water and methanol with or without acid as mobile phase at 1.0 ml/min ...

In this article, we describe a fast and specific method to measure 5FU with HPLC tandem-mass spectrometry. Reversed-phase HPLC was combined with electrospray ionization tandem mass spectrometry and detection was performed by multiple-reaction monitoring. Stable-isotope-labeled 5FU (1,3-15N2-5FU) was used as an internal standard. 5FU was measured within a single analytical run of 16 min with a l...

A simple, selective, rapid and precise reverse phase HPLC method has been developed for the simultaneous estimation of Ofloxacin and Satranidazole in tablets. The analyte was resolved by using a mobile phase 0.05 M phosphate buffer and acetonitrile in the ratio of 65:35 v/v at a flow rate of 1.0 ml/min on an isocratic HPLC system at a wavelength of 320 nm. The linearity was obtained in the conc...

Chemometrics-assisted UV spectrophotometric and RP-HPLC methods are presented for the simultaneous determination of tolperisone hydrochloride (TOL) and diclofenac sodium (DIC) from their combined pharmaceutical dosage form. Chemometric methods are based on principal component regression and partial least-square regression models. Two sets of standard mixtures, calibration sets, and validation s...

Herein, I describe pyridylamination for versatile analysis of sugar chains. The reducing ends of the sugar chains are tagged with 2-aminopyridine and the resultant chemically stable fluorescent derivatives are used for structural/functional analysis. Pyridylamination is an effective "operating system" for increasing sensitivity and simplifying the analytical procedures including mass spectromet...

Abstract A homochiral metal–organic framework (MOF) comprising [Co(L)(bpe)2(H2O)2]·H2O was prepared using (1R,2R)-(−)-1,2-cyclohexanedicarboxylic acid (H2L) and 1,2-bis(4-pyridyl)-ethylene as organic ligands. The MOF explored chiral stationary phase (CSP) for high-performance liquid chromatography (HPLC) separation of racemates. Nine racemates including naphthol, alcohol, diol, amine, ketone, e...

This paper describes the determination of fatty acid composition of coffee, citrus and rum distillery wastes using reversed-phase high-performance liquid chromatography (RP-HPLC). Lipid extracts of the waste samples are derivatized with phenacyl bromide and their phenacyl esters are separated on a C8 reversed-phase column by using continuous gradient elution with water and acetonitrile. The pre...