Glomerular inositol content and the turnover of polyphosphoinositides was reduced by 58% in 1-2 wk streptozotocin diabetic rats. Addition of inositol to the incubation medium increased polyphosphoinositide turnover in glomeruli from diabetic rats to control values. Despite the reduction in inositol content and polyphosphoinositide turnover, protein kinase C was activated in glomeruli from diabe...

As in most invertebrate microvillar photoreceptors, phototransduction in Drosophila melanogaster uses a G-protein-coupled phosphoinositide pathway, whereby hydrolysis of phosphatidyl inositol 4,5-bisphosphate (PIP(2)) by phospholipase C generates inositol 1,4,5-trisphosphate (InsP(3)) and diacyl glycerol (DAG), leading to activation of two classes of Ca(2+)-permeable light-sensitive channel, en...

Polar lipids are important membrane components of plant cells. They are known to affect certain membrane properties like permeability, fluidity and active transport. In the present study, individual leaf buds were tagged on the day of appearance and sampling began 7 days later. Additional samples were taken at 7 days intervals until leaf abscission occurred about 77 days after tagging. The expe...

1. Lysosomes from rat liver contain two enzymic systems for hydrolysing phosphatidyl-inositol: a deacylation via lysophosphatidylinositol producing glycerophosphoinositol and non-esterified fatty acid, and a phospholipase C-like cleavage into inositol 1-phosphate and diaclygycerol. 2. The separate enzyme systems involved can be distinguished by gel filtration, differential temperature-stability...

THE RAPID turnover of phosphatidate (PhA) and phosphatidyl inositol (PhI) in neural tissue has been related to synaptic function by a number of observations. Increased incorporation of labelled phosphate into these lipids has been reported when cholinergic agents are added to incubated slices of brain (BROSSARD and QUASTEL, 1963; HOKIN and HOKIN, 1955), to homogenates (REDMAN and HOKIN, 1964; D...

Cells of Sulfolobus acidocaldarius contain about 2.5% total lipid on a dry-weight basis. Total lipid was found to contain 10.5% neutral lipid, 67.6% glycolipid, and 21.7% polar lipid. The lipids contained C(40)H(80) isopranol glycerol diethers. Almost no fatty acids were present. The glycolipids were composed of about equal amounts of the glycerol diether analogue of glucosyl galactosyl diglyce...

The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2....

The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme ...

The effect of anti-follicular stimulating hormone (FSH) on testicular lipid and the specific activity of testicular enzymes of the isocitrate dehydrogenase (ICDH), pyruvate/malate dehydrogenase (MDH) and malic enzyme involved in lipogenesis were studied in mature bonnet monkeys, Macaca radiata. Immunization of monkey with anti-FSH for 24 days did not produce any significant changes in the body ...