Abstract Protothecosis is an infectious disease caused by organisms currently classified within the green algal genus Prototheca . The can manifest as cutaneous lesions, olecranon bursitis or disseminated systemic infections in both immunocompetent and immunosuppressed patients. Concerning diagnostics, taxonomic validity important. , closely related to Chlorella species complex, known be polyph...

Abstract. Djoemharsjah IS, Ambarwati R, Rahayu DA. 2023. DNA barcoding Clithonsp. (Gastropoda: Neritidae) from Badur Beach, Madura, Indonesia, based on COI gene molecular marker. Biodiversitas 24: xxxx. Neritidae is polymorphic with various shell colors and patterns; therefore, they were doubts about the identifying species genus Clithon, namely Clithon sp. Beach of Madura Island, Sumenep Distr...

Abstract Phylogenetic analyses can be used to resolve taxonomic uncertainties and reconstruct a species’ evolutionary history. This combined with ecological data predict missing life history traits which are important for creation of conservation management strategies. We investigated the ‘Critically Endangered’ Grenada Dove Leptotila wellsi by estimating its phylogenetic placement using this n...

Marker-assisted background selection is a powerful molecular tool that can enhance breeding efficiency through the analysis of large number markers representing entire genomic for precise selection. In present study, transcriptomes 38 cucumber inbred lines with diverse traits were sequenced single nucleotide polymorphism (SNP) mining practical application to commercial breeding. A total 62,378 ...

The internal transcribed spacer 2 (ITS2) has been used as a phylogenetic marker for more than two decades. As ITS2 research mainly focused on the very variable ITS2 sequence, it confined this marker to low-level phylogenetics only. However, the combination of the ITS2 sequence and its highly conserved secondary structure improves the phylogenetic resolution(1) and allows phylogenetic inferenc...

We present a strategy for phylogenetic marker development in non-model systems. Rather than using the traditional approach of comparing distantly related taxa to develop conserved primers for unknown species, we explore an alternative strategy that builds primers directly from a single, relatively well characterized species and applies those primers to increasingly distantly related taxa. We de...

The species-flocks of cichlid fishes in the East African Great Lakes Victoria, Malawi and Tanganyika constitute the most diverse extant adaptive radiations in vertebrates. Lake Tanganyika, the oldest of the lakes, harbors the morphologically and genetically most diverse assemblage of cichlids and contains the highest number of endemic cichlid genera of all African lakes. Based on morphological ...

Five novel halophilic archaeal strains, named BND22 T , ZY10 ZY41 ZY58 and ZY43 were isolated from the coastal saline sediment of intertidal zone located in Qingdao natural sea salt produced Huanghua marine solar saltern, PRChina. These five strains demonstrated typical morphology growth characteristics haloarchaea. The comparison 16S rRNA gene revealed that strain BDN22 was associated with Sal...

The application of nuclear rDNA ITS sequences in plant phylogeny and evolution analysis was discussed in this paper. Nuclear rDNA is an important nuclear gene fragment, which had been widely used in phylogenetic studies. The internal transcribed spacer of nuclear rDNA was divided into ITS1 and ITS2 by 5.8S rDNA. The length of ITS1 ranges from 165 bp to 298 bp in angiosperms, and the length of I...