rice bran (rb) is a major by-product, produced from the rice milling process. this study was conducted to evaluate the performance, egg quality, and blood biochemical parameters of 288 hy-line laying hens fed diets with rice bran and commercial microbial phytase natuphos®5000. the experiment was a 4×2 factorial one with four rb levels (0, 5, 10, and 15%) by two phytase levels (0 and 5000 ftu/kg...

STANCHEV, V., D. GEORGIEV and S. GARGOVA, 2010. Mathematical modeling of the nutrient medium composition for the production of yeast phytase. Bulg. J. Agric. Sci., 16: 628-634 The objective of the present study was the optimization of the nutrient medium composition for Candida melibiosica 2491 cultivation by means of mathematical methods of modeling and production of yeasts with higher enzyme ...

Energy utilization in broilers as influenced by supplementation of enzymes containing phytase or carbohydrase activities was investigated. Day-old male broilers (480) were allocated to four slaughter groups, thirty broilers in the initial slaughter group and 150 broilers in each of the final slaughter groups on days 7, 14 and 21. Broilers in each of the final slaughter groups were allocated to ...

A study was conducted to determine the efficacy of phytase, an enzymic cocktail, and a waste Aspergillus niger mycelium to hydrolyze phytate present in corn-soybean meal diets. One hundred turkey poults were assigned to dietary treatments for 2 wk (days 7 to 21). Dietary treatments included: 1) NRC (1994) diet (NRC), with recommended concentration of 0.6% available P (aP) and 1.2% Ca; 2) Phytas...

Amino acid sequence analysis of fungal histidine acid phosphatases displaying phytase activity has revealed a conserved eight-cysteine motif. These conserved amino acids are not directly associated with catalytic function; rather they appear to be essential in the formation of disulfide bridges. Their role is seen as being similar to another eight-cysteine motif recently reported in the amino a...

The effect of phytase enzyme on the digestibility and availability of phosphorus (P) in soya-bean meal was investigated with grower pigs. In addition, the relative effectiveness of different parameters for assessing P availability in slope-ratio assays were compared. The addition of phytase increased growth rate (741 vs 835 g/d; P c 0.05>, lowered the FCR (2.37 vs 2.16; P c O.Ol), increased pro...

Phytic acid is a main reservoir of phosphorous (P) in plants and contributes to about 80% of the total P in cereal seeds. However, it is well known to possess anti-nutritional behavior. Because it has strong affinity to chelate divalent ions e.g. calcium, magnesium, and especially with iron and zinc. Therefore, it is extremely poor as a dietary source of P. To enhance bio-availability of micron...

Screening throughput is a key in directed evolution experiments and enzyme discovery. Here, we describe a high-throughput screening platform based on a coupled reaction of glucose oxidase and a hydrolase (Yersinia mollaretii phytase [YmPh]). The coupled reaction produces hydroxyl radicals through Fenton's reaction, acting as initiator of poly(ethyleneglycol)-acrylate-based polymerization incorp...

Arsenate interferes with enzymatic processes and inhibits inorganic phosphorus (Pi) uptake in many plants. This study examined the role of phytase and phosphatase in arsenate tolerance and phosphorus (P) acquisition in the arsenic hyperaccumulator Pteris vittata . Enzyme-mediated hydrolysis of phytate in P. vittata extracts was not inhibited by arsenate at 5 mM or by heating at 100 °C for 10 mi...

BACKGROUND In plant-derived animal feedstuffs, nearly 80 % of the total phosphorus content is stored as phytate. However, phytate is poorly digested by monogastric animals such as poultry, swine and fish, as they lack the hydrolytic enzyme phytase; hence it is regarded as a nutritionally inactive compound from a phosphate bioavailability point of view. In addition, it also chelates important di...